Reply To: Scripts 2014

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    Date:
    July 18, 2014
    Source:
    American Gastroenterological Association
    Dietary elimination is a successful method of treatment for adults with eosinophilic esophagitis (EoE), according to a new study in Clinical Gastroenterology and Hepatology, the official clinical practice journal of the American Gastroenterological Association.—“By eliminating specific foods from patients’ diets, symptoms improved in 71 percent of patients, and endoscopic appearance improved in 54 percent,” said lead study author, W. Asher Wolf, MD, MPH, and co-author Evan S. Dellon, MD, MPH, from the division of gastroenterology and hepatology, University of North Carolina School of Medicine. “These strong results support dietary elimination therapy as an effective treatment for adults suffering from EoE.”–Researchers conducted a retrospective cohort study using the University of North Carolina EoE database from 2006-2012. Subjects were 18 years of age or older and diagnosed with EoE based on consensus guidelines. The study analyzed two diet elimination plans. For the first therapy — targeted elimination — patients underwent evaluation with skin prick testing, and foods that had a positive reaction, as well as any foods identified by patient self-report as being possible triggers regardless of the severity of response, were eliminated. For the other diet prescribed — the six-food elimination diet — dairy, wheat, eggs, soy, nuts and seafood were eliminated from the patient’s diet regardless of skin-prick test results.—Sixty-eight percent of patients who received targeted diet elimination experienced symptom improvement, compared with 78 percent of those who followed a six-food elimination diet plan. For those subjects who responded to six-food elimination diet, one food (or food group) was added back every six weeks, and endoscopy was repeated. Eggs and dairy were found to be the most common triggers, both impacting 44 percent of patients.—[F5]–Importantly, because this study included patients who failed steroid therapy, the overall study population likely represents harder to treat EoE patients. The success of dietary elimination in this population indicates its utility for therapy in patients resistant to steroids.–Because EoE is an allergen/immune mediated condition, dietary elimination therapy has been extensively studied in children and is now a well-established modality in pediatric patients. Until recently, the utility of dietary elimination was unknown in adults. However, evidence is mounting; a June 2014 Gastroenterology meta-analysis2 reported that dietary interventions are effective in producing histologic remission in both pediatric and adult patients with EoE.
    While the current treatment of swallowed corticosteroids acts topically to reduce esophageal inflammation, no corticosteroid is FDA approved, not all patients respond and, when discontinued, EoE almost always recurs. As such, there is a growing need for a new treatment for patients with EoE.–Further research should emphasize which factors can predict effective dietary therapy, to target therapy to patients most likely to respond.-Story Source–The above story is based on materials provided by American Gastroenterological Association. Note: Materials may be edited for content and length.–Journal Reference-W. Asher Wolf, Maya R. Jerath, Sarah L.W. Sperry, Nicholas J. Shaheen, Evan S. Dellon. Dietary Elimination Therapy Is an Effective Option for Adults With Eosinophilic Esophagitis. Clinical Gastroenterology and Hepatology, 2014; 12 (8): 1272 DOI: 10.1016/j.cgh.2013.12.034
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    The role of dairy in maintaining adult bone and skeletal muscle health
    Date:
    July 28, 2014
    Source:
    Taylor & Francis
    Understanding that diets are often built around food groups rather than specific nutrients, researchers from Switzerland, France, and North America decided to examine interactions between four nutrients found in dairy products and their role in preserving bone and skeletal muscle. Their Open Access article with these findings, “Dairy in Adulthood: From Foods to Nutrient Interactions on Bone and Skeletal Muscle Health,” is now available in the Journal of the American College of Nutrition, the Official Publication of the American College of Nutrition and a publication from Routledge.–Calcium (Ca), inorganic phosphate (Pi), vitamin D, and protein are nutrients that impact bone and skeletal muscle integrity. Deficiency in the supply of these nutrients increases with aging. Dairy foods are rich in Ca, Pi, and proteins and in many countries are fortified with vitamin D. Dairy foods are important sources of these nutrients and go a long way to meeting the recommendations, which increase with aging. This review emphasizes the interactions between these 4 nutrients, which, along with physical activity, act through cellular and physiological pathways favoring the maintenance of both bone and skeletal muscle structure and function[F6].–While bone health is often associated with calcium alone, Calcium’s interactions with inorganic phosphate, vitamin D, and protein are important components of beneficial dairy consumption. Combined vitamin D and calcium supplementation has been shown to reduce the incidence of hip and other non-vertebral fractures among older populations, with some studies suggesting that vitamin D actually leads to lower rates of falling in subjects. Dietary protein, while often associated solely with muscle recovery, also promotes bone formation by stimulating both Ca and Pi intestinal absorption and the production of a bone growth factor. And while vitamin D deficiency is associated with muscle weakness, vitamin D and protein supplementation are required together to improve strength.–Combining the four above-mentioned nutrients with physical activity decreases the likelihood of bone and muscle degeneration-related injury in older adults. Dairy products are a convenient way to work them into a diet, as they contain Ca, Pi, and protein at levels comparable to recommended intakes, and are fortified with vitamin D.–Story Source-The above story is based on materials provided by Taylor & Francis. Note: Materials may be edited for content and length.–Journal Reference-Jean-Philippe Bonjour, Marius Kraenzlin, Régis Levasseur, Michelle Warren, Susan Whiting. Dairy in Adulthood: From Foods to Nutrient Interactions on Bone and Skeletal Muscle Health. Journal of the American College of Nutrition, 2013; 32 (4): 251 DOI: 10.1080/07315724.2013.816604
     
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    Milk does a Body Good- we are going to take dairy and show how to use this to make it better and wholesome— Now the best milk to consume is Unpasteurized Milk —the store bought homogenized is basically a leftover milk byproduct—and not even milk—Now if you cannot get Unpasteurized Milk due to Gov’t Stupidity—and regulations—then utilize kefir or yogurt or buttermilk with fat—the effects will not be as pronounced but will still benift
    Here are some remedies to try to build and restore the body
     
    ùFor children take full fat yogurt or kefir 16 oz added to a blender add 1 tsp of gelatin and 1 tablespoon of coconut oil or mct oil—adding wheat germ oil would be good as well—mix in blender and when down ad 1 drop of iodine to the mix after and stir in with either a wooden spoon or chopstick—consume
     
    ùIf you can get unpasteurized milk 16 oz then add 1 egg ( farmer bought –and orange or reddish yolk) add cinnamon to this and wheat germ oil blend til mixed then after add your iodine— optional add shilajit or fulvic minerals to this to further make these minerals more readily absorbable
     
    ùButtermilk—add glycerol and shila jit-leucine about ¼ tsp and lipase enzyme ¼ tsp—blend together —this will again more readily taken in
     
    ùCottage Cheese –full fat 16 oz add to it berries and minerals capsule(s) —B complex 1-3 capsules-and some berry( of choice—avoid strawberries unless home grown or chemical free) add distilled water if necessary and blend
     
    ùùin any of these combinations have a potato with this –broiled—baked or mashed—and this will be a complete meal
     
    ùùùif you have no choice and are stuck then use powdered milk—add the gelatin and fat to this and minerals and b vitamins to make your self a real milk product
     
    ùùCanned Milk can be utilized as well –by again adding fat and nutrients—
     
    ùto increase Phosphorus—the egg will do this —sunflower leicithin—or even TSP—if you wish to flavour this then add vanilla or almond extract as some suggestions—to increase fat heat the butter scoop out the cream and add the oil
    and if you wish add your cq 10 to this and vitamin A and D
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    Show of the Month August 9 2014
    Kidney Stones—
     
    Teeth Restore
    Fat—what to use when doing ketosis
    Toxic –cleaning agents that are supposed to be Dangerous
     
    Hydrator internal and external
     
    Liquid diets
    Labourers Health
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    Kidney Stones—
    Citric Acid –will dissolve stones—by using ¼ tsp in water and drunk 4-5 times a day—-the use of magnesium citrate and potasium citrate or a sodium citrate will also assist in the breaking this down—eliminating things like beer-spinach-peanuts-and beets for a period time allowing the kidneys to flush out the crystals
    Using as well distilled water or reverse osmosis to keep the water clean and to allow for flushing and not adding harmful metals
     
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    Teeth Restore
    Use 10 grams of TSP ( 2 tsp) 90 grams( 6 tablespoon)—mixed in 24-30 oz distilled water –dissolve—and use a ¼ cup at a time –can be diluted with tap water if to strong—use as a oil pulling pushing this through the teeth and drawing
    Do not brush—repeat the process 5-7 times—you may see specks of polymers pop out of the mouth—you may see fibres come out—what you may see as well is a reversal of cavity damage and a regrowth—internally you will as well utilize the ascorbate mineral mix to replenish the loss of vitamin C and the use of essential mineral building blocks—magnesium citrate-potassium citrate –boron( borax) TSP ( trisdium phosphate for both sodium and phosphorus) zinc citrate- and baking soda—this done internally will assist in the materials required to upbuild—the elimination of all grains and cereals and breads—and soy are imperative to seeing success—the use of gelatin –will also benefit—nothing with microwave or canola and soybean oils since they are destructive to thyroid—and pancreas stomach-thymus—process foods are to be avoided as well
     
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    Fat—what to use when doing ketosis—
    Lipase should be incorporated with any fats to assist to break down and conversion of hormones and the use of fats for cellular health and energy for the organs— other things to consider as well would be B5-for energy and fat use and hormonal production—Choline-to regulate the fat usage –L carnitine to convert the fat for energy production- taurine to increase Bile salt production- and sunflower or egg yolk lecithin to control the flow of fat as well—other supplements you may want to take with fat are cq10-Vitamin A-( strongly encourage) Vitamin D ( minimal amounts 400-1000IU) Vitamin E( none soy sourced) essential oil of rosemary-sage-thyme-orange- tangerine-BHT as antioxidants to slow down the break down of the fat so it remains effective longer in the body before it becomes periodized( broken down) and the use of rosemary-bay leaf- thyme-garlic-onion-sage-to again assist in the sustaining the fat and the uses
     
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    Toxic –cleaning agents that are supposed to be Dangerous
     
    TSP –sodium and phosphorus
    Ascorbic Acid- Vitamin C derivative-antioxidant
    Vinegar—Anti mold –Kills HIV –and is used as an industrial Cleaner
    Iodine—body support overall –used as an industrial cleaner
    Turpentine-essential oil of pine-balsaam fir-spruce—Potent antioxidants-supports male endocrine system –kills off harmul pathogens in the body
    Used as an industrial cleaner to sanitize and to remove vermin
    Colloidal Silver-Colloidal Copper—high powered anti fungals-anti algaeicides-anti bacterial—anti microbial-anti virals—used in the medical settings as industrial cleaning agents
    Hydrogen Peroxide – pro oxidant—antioxidant—used as an industrial cleaner to remove a host of conditions
    MMS- sodium chlorite used as a industrial cleaner to kill off all kinds of pathogenic infections
    Baking Soda—used as a cleaning agent –used in the body to remove fungal over growth –cancer eliminator and digestive aide
    Thyme and Mint- have been used as a cleaning and sanitizing agents but when and as a oral sanitizer
    White Wine—used as an industrial cleaning agent used to reduce or clean Mold
    STS-Sodium Thiolsulphate—used as an industrial cleaner to remove chlorine and used in photography—and paper industry—used as a potent chelating agent for the body removing harmful build up of chemical dust and metals and radioactivity
    EDTA—used as an industrial cleanser in removing metal ions—and is used as well for chelating metals and some chemicals in the Body
    Borax-used as a cleaning agent and detergent additive—is also used by the body to heal and regenerate and combines with magnesium to assist with bone health and regulates hormones
     
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    Hydrator internal and external—when going outside and are being exposed to Microwave heat and Hot days and Chemtrails this can have a frying effect on the system allowing the cells to become saturated with the fall out and radiation –so what you want to do is to take glycerin or glycerol and mix lugols iodine with this and apply topically to the skin—take 1 oz of glycerol 10-20 drops of iodine and mix well—you can screw the top of the container if you put it in a 1 oz seal top and either chake or percuss about 100 times the apply—you can wet the areas first with water and then apply allowing this to dry on it’s own sealing the cells with the water and the iodine and glycerol— you can take a tsp of this orally—you can modify by adding TSP –or other electrolytes with the iodine as well —this will alow for sustained hydration-ATP production( energy for the cells) and electrolyte and hydration not to mention anti radiation effect of the iodine
     
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    Liquid diets— what to do to safely clear out and nourish and build with a compromised digestive system—so what would you use—here are several things you can use to assist you—Meat Extracts—take meat—lean—puncture the meat or slice it –then add to a jar—and lightly seal the jar –add to a pot and allow to boil or high simmer for several hours and then when done pour the liquid content to a jar- season with salt if you wish and use tsp increments—Juices utilze and veg or fruit juice and when done add some gelatin to this to increase the mineral uptake and protein along with the antioxidant and vitamin content—an alternative is to allow the juices to break down and ferment so that the sugars can be reduced and the mineral or antioxidant content can increase allowing for better uptake of the nutrients-this will assist in restoration in a lot of areas—Gelatin broths —heating water and dissolving gelatin in distilled water and when dissolved utilize as a beverage—can and herbals as well as minerals with this combination to rebuild muscle-organ-bone and tissue—Fermented Dairy—yogurt-kefir-sour creams—cheese—can be utilized as well in the repairing and regenerating the immune system—repairing the colon—regrowing a colon—cleansing the system and utilizing minerals and proteins and fas more efficiently —can utilize sugars as well to increase bacterial levels—will see butyric acds and lactic acids for killing off cancers — Teas and Herbal Fusions—making teas and using either one or several herbs at a time to increase the desired effect —will release minerals—as well as antioxidants—and vitamins—depending on the combination some will just steep others will boil—Soups which are essential fusions but with a mix of protein and carbs and veges are another way to increase nutrients in a way where one is compromised can see vitality levels return- Protein Drinks or Smoothies can also be utilized as a means of getting high dense nutrients into the body—by adding a fermented dairy with this —can see a more readily use and uptake of the proteins and other minerals mixed with this combination
     
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    Labourers Health— this one is going to be diverse but you will have to utilize the basics to assist you with your daily labours—the one thing all labours need are antioxidants—and the cheapest and most effective of them will be Ascorbic Acid and Sulphur—and the easiest Sulphur is MSM—and the other important power vitamin is B1—this combination can remove toxic build up of industrial and environmental pollutants that labourers are exposed to
    The minimum dail dose is about 1 gram-1000mgs-1/4 tsp of Ascorbic acid with ¼ tsp of MSM and 100mgs of B1 taken several timesa day usually a minimum of 5 times a day—this may see recovery as well as healing and cleansing effect of the toxins—Food wise depending on the type of labour—should be Protein—then Fat—then fruit or vegs—Breads—Cereals—Pastas—Rice-Corn will be counter productive—consuming either onion—or garlic or ginger with the protein whether in drink as a tea or a juice or mixed with foods will increase much needed Testosterone for men and some for women which will incorporate the proteins more effectively in the muscles—and fruit –berries—cherries—citrus fruits which will have a good mineral content of magnesium and potassium –any apple or pear—or fruits in general—the ones to avoid are banaas –grapes-raisons –watermelon and dates—these have to a high glycemic rate and can be problematic –fats to utilize are the saturated fats—for long term power and energy for the muscles to work—incorporating lipase-to insure usage-B5 – Taurine- Choline-L carnitine will insure the use of the fats for energy –taking these daily in 250-500mgs with meals will increase the use—the lipase being the exception—only need between this can be taken in increments of 1-4 hundred mgs- to insure a good break down of the fats—which can increase fecal volume leaving the tract—the consumption of yogurt and kefir and cottage cheese and cheeses can also increase probiotic activity as well as protein and fats required for stamina and strength—these can be utilized as well
     
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    [F1]Iodine deficiency would appear to be a cause of eye swelling
    [F2]Alcohol Extracts
    [F3]within the living
    [F4]suggested dose
    [F5]The question is what type of dairy and what type of egg—this is where this gets sketchy and the bias in favor of soy again is being pushed—soy and casein will have the exact same immune triggering response irregardless of the person and if the soy contamination has not had a thorough washout period then there will be a reaction irrespective of what you eat
    [F6]Would be in today’s time Unpasteurized Milk not processed —if processed the effect will be at best nominal—with the contaminants
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    Show of the Month August 16 2014
    Effect of icariin on osteoblastic differentiation gene expression of human periodontal ligament cells
    Whey to control diabetes
    Chili peppers for a healthy gut- Spicy chemical may inhibit gut tumors
    Cytotoxicity and Inflammatory Effect of Silver Nanoparticles
    in Human Cells
    Green synthesis of silver nanoparticles for the control of mosquito vectors of malaria, filariasis, and dengue
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    Effect of icariin on osteoblastic differentiation gene expression of human periodontal ligament cells.
    Beijing Da Xue Xue Bao. 2013 Dec 18;45(6):975-8
    Authors: Ding Q, Zhang FQ, Ma YS
    Abstract
    OBJECTIVE: To observe the effect of icariin on human periodontal ligament cells (hPDLCs) differentiation to osteoblast gene expression.
    METHODS: The fifth generation of the cultured hPDLCs was added with the concentration of 0.01 mg/L icariin, and the added osteogenic medium used as blank control group, alizarin red staining of icariin on human periodontal ligament cells was observed for 21 days; the 2, 4, and 6 days of Q-PCR quantitative analysis of icariin on human periodontal ligament cells were made for osteogenesis gene alkaline phosphatase (ALP), type I collagen and osteocalcin (OC) gene expression.
    RESULTS: For the 21 days, alizarin red staining icariin group formed more mineralized nodules; on the 2nd, 4th, and 6th days, the group of icariin promoted the expression of ALP and OC mRNA, reached the peak value on day 6, compared with the control group with significant difference (20.15±6.67 vs. 7.90±0.71, 4.13±0.56 vs. 3.55±0.08, P<0.01). The second day, the highest expression of type I collagen appeared, then decreased gradually after, statistically compared with the control group (P<0.05).–CONCLUSION: Icariin can promote the human periodontal ligament cells differentiation to osteoblast, and promote the osteogenesis gene expression.–PMID: 24343085 [PubMed – indexed for MEDLINE]
    Recipe— Take a alcohol extract and apply direct on the gums or utilize as a mix to swish in the mouth
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    Whey to control diabetes
    Date:
    August 5, 2014
    Source:
    American Friends of Tel Aviv University
    Blood sugar surges — after-meal glucose “spikes” — can be life threatening for the 29 million Americans with diabetes. Diabetic blood sugar spikes have been linked to cardiovascular disease, cancer, Alzheimer’s disease, kidney failure, and retinal damage. Now a new Tel Aviv University study, published in Diabetologia, suggests a novel way to suppress these deadly post-meal glucose surges: the consumption of whey protein concentrate, found in the watery portion of milk separated from cheese curds, before breakfast.–According to TAU’s Prof. Daniela Jakubowicz and Dr. Julio Wainstein of the Wolfson Medical Center’s Diabetes Unit, Prof. Oren Froy of the Hebrew University of Jerusalem, and Prof. Bo Ahrén of Lund University in Sweden, the consumption of whey protein before meals may even keep diabetics’ need for insulin treatment at bay.–“What’s remarkable is that consuming whey protein before meals reduces the blood sugar spikes seen after meals. It also improves the body’s insulin response, putting it in the same range or even higher than that produced by novel anti-diabetic drugs,” said Prof. Jakubowicz. “High milk intake has long been associated with lower risk for type 2 diabetes and cardiovascular disease,[F1] and milk whey protein increases the production of a gut hormone called glucagon-like peptide-1 (GLP-1) that stimulates insulin secretion. This, in turn, reduces the blood glucose rise after meals.”-“We hypothesized that stimulating GLP-1 production by consuming whey protein before a meal would enhance insulin secretion and have beneficial glucose-lowering effects in type 2 diabetes,” Prof. Jakubowicz said.——–The study was conducted on 15 individuals with well-controlled type 2 diabetes at Wolfson Medical Center. The participants were randomized to receive either 50 grams of whey in 250 ml water or a placebo,[F2] followed by a standardized high-glycemic index breakfast of three slices of white bread and sugary jelly — a meal designed to produce the maximum post-meal glucose spike.—Blood samples were taken 30 minutes before the meal, when the whey protein or placebo drinks were consumed. Further blood samples, assessing plasma concentration of glucose, intact GLP-1, and insulin concentrations, were taken when the breakfast was served and at 15, 30, 60, 90, 120, 150, and 180 minute intervals after the meal.—–The researchers found that glucose levels were reduced by 28 percent after the whey pre-load over the 180-minute post-meal period, with a uniform reduction during early and late phases. With whey pre-load, insulin and GLP-1 responses also were significantly higher (105 and 141 percent, respectively), producing a 96 percent increase in early insulin response.—“The early insulin response that usually is deficient in type-2 diabetes was significantly higher after whey protein than with placebo, and the whey protein preload significantly reduced the elevation of blood glucose after breakfast,” said Prof. Jakubowicz. “Whey protein could therefore represent a novel approach for enhancing glucose-lowering strategies in type 2 diabetes.”–Based on the findings of this study, the authors are considering a long-term clinical trial to test the enduring benefits of whey protein consumption for diabetics.–Story Source–The above story is based on materials provided by American Friends of Tel Aviv University. Note: Materials may be edited for content and length.–Journal Reference-Daniela Jakubowicz, Oren Froy, Bo Ahrén, Mona Boaz, Zohar Landau, Yosefa Bar-Dayan, Tali Ganz, Maayan Barnea, Julio Wainstein. Incretin, insulinotropic and glucose-lowering effects of whey protein pre-load in type 2 diabetes: a randomised clinical trial. Diabetologia, 2014; 57 (9): 1807 DOI: 10.1007/s00125-014-3305-x
    Suggestion—When Buy a whey product there will be several—whey concentrate-whey isolate-whey hydroslate —in this study the whey concentrate was used because of it is slower release and not used up as quickly—the isolates and hydroslates—are uptaken very easily and can e regulated with the use of fats—
    Never use any whey mixed with aceslfame-aspartame-neotame-artificial flavour-natural flavour-HFCS-Corn Sugar-SOY—-it should be a plain whey—with no additive—flavours can be real vanilla and real cocoa—or buy plain and add this to the smoothie or protein mix
     
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    Chili peppers for a healthy gut- Spicy chemical may inhibit gut tumors
    Date:
    August 1, 2014
    Source:
    University of California, San Diego Health Sciences
     
    Chili peppers.
    Credit: © Alexey Laputin / Fotolia
    Researchers at the University of California, San Diego School of Medicine report that dietary capsaicin — the active ingredient in chili peppers — produces chronic activation of a receptor on cells lining the intestines of mice, triggering a reaction that ultimately reduces the risk of colorectal tumors.–The findings are published in the August 1, 2014 issue of The Journal of Clinical Investigation.–The receptor or ion channel, called TRPV1, was originally discovered in sensory neurons, where it acts as a sentinel for heat, acidity and spicy chemicals in the environment. “These are all potentially harmful stimuli to cells,” said Eyal Raz, MD, professor of Medicine and senior author of the study. “Thus, TRPV1 was quickly described as a molecular ‘pain receptor.’ This can be considered to be its conventional function, which all takes place in the nervous system.”–But Raz and colleagues have found that TPRV1 is also expressed by epithelial cells of the intestines, where it is activated by epidermal growth factor receptor or EGFR. EGFR is an important driver of cell proliferation in the intestines, whose epithelial lining is replaced approximately every four to six days.—“A basic level of EGFR activity is required to maintain the normal cell turnover in the gut,” said Petrus de Jong, MD, first author of the study. “However, if EGFR signaling is left unrestrained, the risk of sporadic tumor development increases.”—The scientists discovered that TRPV1, once activated by the EGFR, initiates a direct negative feedback on the EGFR, dampening the latter to reduce the risk of unwanted growth and intestinal tumor development. They found that mice genetically modified to be TRPV1-deficient suffered higher-than-normal rates of intestinal tumor growths.–“These results showed us that epithelial TRPV1 normally works as a tumor suppressor in the intestines,” said de Jong. In addition, molecular studies of human colorectal cancer samples recently uncovered multiple mutations in the TRPV1 gene, though Raz noted that currently there is no direct evidence that TRPV1 deficiency is a risk factor for colorectal cancer in humans.–“A direct association between TRPV1 function and human colorectal cancer should be addressed in future clinical studies,” he said.–But if such proves to be the case, the current study suggests one potential remedy might be spicy capsaicin, which acts as an irritant in mammals, generating a burning sensation in contact with tissue. Capsaicin is already broadly used as an analgesic in topical ointments, where its properties as an irritant overwhelm nerves, rendering them unable to report pain for extended periods of time. It’s also the active ingredient in pepper spray.
    The researchers fed capsaicin to mice genetically prone to developing multiple tumors in the gastrointestinal tract. The treatment resulted in a reduced tumor burden and extended the lifespans of the mice by more than 30 percent. The treatment was even more effective when combined with celecoxib, a COX-2 non-steroidal anti-inflammatory drug already approved for treating some forms of arthritis and pain.—“Our data suggest that individuals at high risk of developing recurrent intestinal tumors may benefit from chronic TRPV1 activation,” said Raz. “We have provided proof-of-principle.”–Co-authors include Petrus R. de Jong, UCSD Department of Medicine and University Medical Center Utrecht, The Netherlands; Naoki Takahashi, UCSD Department of Medicine and Niigata University Graduate School of Medical and Dental Sciences, Japan; Alexandra R. Harris, Jihyung Lee, Samuel Bertin, James Jeffries, Michael Jung, Jen Duong, Amy I. Triano, Jongdae Lee, David S. Herdman, Hui Dong, Lars Eckmann and Maripat Corr, UCSD Department of Medicine; Yaron Niv, Rabin Medical Center and Tel Aviv University, Israel; Koji Taniguchi, UCSD Department of Medicine and Keio University School of Medicine, Japan; Chang-Whan Kim, UCSD Department of Medicine and Catholic University of Korea; Stephanie M Stanford and Nunzio Bottini, La Jolla Institute for Allergy and Immunology.—This research was supported, in part, by the Crohn’s and Colitis Foundation of America, the Prins Bernhard Cultural Foundation, the Scholten-Cordes Foundation, the Dr. Hendrick Muller Vaderlandsch Foundation, the Japan Society for the Promotion of Science, the European Molecular Biology Organization, the Juvenile Diabetes Research Foundation, the Strategic Young Researcher Overseas Visits Program for Accelerating Brain Circulation, the Broad Medical Foundation and the National Institutes of Health (grants AI095623 and DK35108).–Story Source-The above story is based on materials provided by University of California, San Diego Health Sciences. Note: Materials may be edited for content and length.–Journal Reference–Petrus R. de Jong, Naoki Takahashi, Alexandra R. Harris, Jihyung Lee, Samuel Bertin, James Jeffries, Michael Jung, Jen Duong, Amy I. Triano, Jongdae Lee, Yaron Niv, David S. Herdman, Koji Taniguchi, Chang-Whan Kim, Hui Dong, Lars Eckmann, Stephanie M. Stanford, Nunzio Bottini, Maripat Corr, Eyal Raz. Ion channel TRPV1-dependent activation of PTP1B suppresses EGFR-associated intestinal tumorigenesis. Journal of Clinical Investigation, 2014; DOI: 10.1172/JCI72340
    Suggestions
    utilize in foods—make a straight extract –using either water or alcohol,
    and apply it as a remedy straight usage or add to foods
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    Cytotoxicity and Inflammatory Effect of Silver Nanoparticles
    in Human Cells
     
     
    Cytotoxicity and Inflammatory Effect
    of Silver Nanoparticles
    in Human Cells
    Jeong-shin Park, Na Mi Yu, Jinwoo Cheon and In-Hong Choi
    Department of Microbiology, College of Medicine;
    Department of Chemistry;
    Nanomedical NCRC, Yonsei University, Seoul, Korea
     
    1. Approaches to practical toxicology tests
    to assess nanoparticles
    2. Cytotoxicity and inflammatory effects
    of silver nanoparticles
     
    Nanoparticles and toxicity assay
     
    The rapidly developing field of nanotechnology will result
    in exposure of nanoparticles to humans via several routes
    (e.g., inhalation, ingestion, skin, etc.). Nanoparticles can
    translocate from the route of exposure to other vital
    organs and penetrate cells.
    • Toxicity studies to determine the deleterious effects of
    nanoparticles on living cells are required.
    • Due to the nanosize and the nature of agglomeration,
    simple standard methods to characterize the biological
    effects of nanoparticles are currently unavailable.
    • In this study, practical information regarding the optimal in
    vitro tests for nanotoxicity were evaluated.
     
    Silver nanoparticles
    03/19
    Antimicrobial applications
     
    Ink
     
    Cosmetics 200nm 200nm 500nm
    20 nm (synthetic)
    180 nm
    (commercial,
    Aldrich)
     
     
     
     
    Biological tests Inflammation ØØ Annexin staining,caspase activation ØØCytokine production,activation of
    Signaling molecule ØØROS ØØCytotoxicityØØ MTT/CCK-8 ØØ Establishment of in vitro toxicity assay ØØ Identification of mechanisms for toxicity and inflammation
    Synthesis
    Production & characterization
    of physical and chemical properties
     
     
    In vitro tests for nanoparticles
     
    ISO/TC229
    • OECD
    • U.S NCL
    Review in vitro
    methods
    • Production of
    diverse particles
    (size, surface)
    • Assess biological
    activities
    Assess toxicity tests
    Understanding of proper
    methods for nanoparticles
    Establish proper
    methods
     
    Exposure routes of nanomaterials
     
     
     
    Skin Respiratory trac Immune System
     
     
     
     
    Cell line
    Origin
    Characteristics
     
     
    Respiratory
    A549
    Lung epithelial
    Proper for cytotoxicity
     
    BEAS-2B
    Bronchial epithelial
     
    Proper for cytokine Production
    Immune
    U937
     
    Macrophage
    Proper for cytotoxicity and
    cytokine production
     
     
    Skin
    SK-Mel
    Skin epithelial
    Proper for cytotoxicity and
    cytokine production
     
     
     
    A375
    Skin epithelial
    Too fast growing
     
     
    Standard toxicology tests and silver nanoparticles
     
    In Vitro
    Immunology
    Properties
    (Blood
    contact
    Hemolysis
    Release of
    hemoglobin
    Standard
    Proper
     
    Complement
    activation
    Activation of C3
    complement
    Standard
    Inappropriate
     
    Leukocyte
    proliferation with
    mitogen
    stimulation
    Standard
    CCK-8
    In Vitro
    Immunology
    (Cell-based
    assays)
    Leukocyte
    proliferation
    Zymosan assay
    Standard
    Proper
    Cytokine
    production
    Standard
    Proper
    Phagocytosis
     
     
    Proper
    Cytokine induction
    Toxicity
    Oxidative stress
    Detection of ROS
    Standard
    CCK-8
    Cytotoxicity (necrosis)
    Cell viability and
    mitochondrial
    integrity
    Standard
    Annexin-V
    Cytotoxicity
    (apoptosis)
    Activation of
    caspase 3
    Standard
     
     
     
     
     
     
     
    Targeting
    Cell
    binding/internalization
    N/S
    N/S
    TEM, confocal
    microscope or other
    methods
     
     
    Characteristics specific to metal nanomaterials
     
    Nanoparticles larger than 100 nm tend to aggregate relatively quickly in vitro when compared to nanoparticles smaller than 100 nm. Fresh samples
    within two weeks after synthesis is recommended for tests.

    Each standard toxicology method must be verified before use. (ex. interference with a specific wavelength, electrophoresis)
     
    Flow chart for nanotoxicity tests
     
    Small –Nano Particle Size 100nm-Large
     
    Small–Analysis Of Biological — Nano Particle Size 100nm
     
    – Particle size
    – Cytotoxicityroperties
    – Apoptosis
    – Cytokine production
    – Hemolysis
    – Leukocyte proliferation
    – ROS production
     
    Large— Analysis of chemical/physical
    properties
    – Aggregation
    – Particle size
     
    Cytotoxicity of silver nanoparticles
     
    20 nm– SK-Mel28 (skin) A375 (skin) A549 (lung)
     
    Summary
     
    In human cells, epithelial cells from skin or lung, and macrophages, 5 nm and 20 nm silver particles induced stronger cytotoxicity and ROS synthesis than 80 nm
    particles did.
    • 5 nm and 20 nm silver particles induced chemokine production, mainly IL-8, MIF and RANTES, while proinflammatory cytokines, IL-1, IL-6 and TNF-α were not induced significantly in the same conditions.
    • Some MAP kinase signaling pathways were activated during exposure to silver nanoparticles at lower —concentrations which do not induce cytotoxicity
     
    The toxicity and inflammatory effects of nanoparticles are dependent on their size. In silver nanoparticles smaller than 20 nm induce cytotoxicity significantly in vitro.
    • Nanoparticles induce inflammatory immune responses at lower concentrations and chemokines are the major cytokines induced at early stages of exposure to silver
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    Green synthesis of silver nanoparticles for the control of mosquito vectors of malaria, filariasis, and dengue.
    Vector Borne Zoonotic Dis. 2012 Mar;12(3):262-8
    Authors: Arjunan NK, Murugan K, Rejeeth C, Madhiyazhagan P, Barnard DR
    Abstract
    A biological method was used to synthesize stable silver nanoparticles that were tested as mosquito larvicides against Aedes aegypti, Anopheles stephensi, and Culex quinquefasciatus. Annona squamosa leaf broth (5%) reduced aqueous 1 mM AgNO₃ to stable silver nanoparticles with an average size of 450 nm. The structure and percentage of synthesized nanoparticles was characterized by using ultraviolet spectrophotometry, X-Ray diffraction, Fourier transform infrared spectroscopy, and scanning electron microscopy methods. The median lethal concentrations (LC) of silver nanoparticles that killed fourth instars of Ae. aegypti, Cx. quinquefasciatus, and An. stephensi were 0.30, 0.41, and 2.12 ppm, respectively. Adult longevity (days) in male and female mosquitoes exposed as larvae to 0.1 ppm silver nanoparticles was reduced by ~30% (p<0.05), whereas the number of eggs laid by females exposed as larvae to 0.1 ppm silver nanoparticles decreased by 36% (p<0.05).–PMID: 22022807 [PubMed – indexed for MEDLINE]
    Commentary
    This is alarming —that NanoSilver is being used as a pesticide—the fallout on this on plant life will also have a negative impact on growth as well and this will as well get into the water table for other insects ( beneficial) will also be impacted—the issue here us how do you clean this up—once it is being sprayed and released how do you collect the particles—this is not a good option –nanoparticle do not respond the same way as base metals—this will be a pollutant being added to the eco system causing chain of events—you have to really read this to understand the actual idea of what is transpiring here—if the bugs are being killed and the larva is not happening —then what is that doing to us—when this gets into a crop—with the glyphosates chelating the minerals out and transporting the metals in like the chemtrail fall out then these nano( metals) will also be incorporated and may eventually cause a sterility in the soil since nano silver will kill off the normal bacteria
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    Show of the Month August 23 2014
    Exclusive- NewLink says Ebola vaccine trial could start in weeks
    Can instant noodles lead to heart disease, diabetes and stroke?
    Coming soon- Genetically edited ‘super bananas’ and other fruit
    Brown fat protects against diabetes, obesity in humans
    Nigella sativa concoction induced sustained seroreversion in HIV patient
    NANO CHEMTRAILS
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    Exclusive- NewLink says Ebola vaccine trial could start in weeks
    Wed, Aug 13 2014
    By Sharon Begley
    (Reuters) – NewLink Genetics Corp, which licensed an Ebola vaccine developed by Canadian government scientists, has enough doses on hand to launch the first human safety trial of an Ebola vaccine this summer[F3], its chief executive said on Wednesday. The company has also lined up two contract manufacturing companies and possibly a third and will be able to produce tens of thousands of vaccine doses within “the next month or two,” Dr Charles Link said in an interview. The largest Ebola outbreak in history, which has killed more than 1,000 people in West Africa[F4], has lent an unprecedented urgency to efforts to develop vaccines and treatments, which for years had largely languished. -Last week, the Ames, Iowa-based company’s wholly owned subsidiary, BioProtection Systems Corp, received $1 million from the United States Defense Threat Reduction Agency (DTRA) for more pre-clinical toxicology studies, including stepped-up manufacturing, to allow human trials to begin quickly. The vaccine was developed by scientists at the Public Health Agency of Canada.[F5] “DTRA said, ‘we want this to move quickly,'” Link said. “Before that, I’d have said it would take eight to 10 months before we could launch human studies, but now it’s a matter of weeks.” Only one treatment, made by Tekmira Pharmaceuticals, had even begun human safety trials,[F6] while the others had been tested only in non-human primates[F7]. In addition to NewLink, pharmaceutical giant GlaxoSmithKline is awaiting approval from the U.S. Food and Drug Administration (FDA) to begin a human safety trial of an experimental vaccine, possibly as soon as next month. The World Health Organization said on Tuesday that two experimental Ebola vaccines were set to enter clinical trials in the coming weeks and that there could be enough early-stage data to consider their emergency use late this year. “There is a way to fast-track clinical trials,” WHO Assistant Director-General Marie-Paule Kieny said. WHO’s interest has acted like a starter’s pistol in the race to get Ebola drugs or vaccines into the field. On Wednesday, Dr. Anthony Fauci, director of the National Institute of Allergy and Infectious Diseases, wrote in an essay in the New England Journal of Medicine that “production scale-up” of ZMapp is under way but will take time. ZMapp is the cocktail of antibodies, produced by Mapp Biopharmaceutical of San Diego, given to two American medical workers who contracted Ebola and were evacuated to Atlanta. Also on Wednesday, Canada’s Tekmira Pharmaceuticals said it was discussing options for its experimental Ebola treatment with governments and other agencies, including the WHO.
    DISCUSSIONS WITH GOVERNMENT
    NewLink is attempting to fast-track its clinical trials.–Link said the company does not have to wait for manufacturing to ramp up before launching a safety trial of its vaccine: “We reserved plenty of doses” from ongoing studies in lab animals “to do the first human studies.” The company is in discussions with the U.S. Centers for Disease Control and Prevention (CDC), the National Institutes of Health and Walter Reed Army Medical Center about where to conduct that trial and how to recruit volunteers. To get robust data, Link said, the vaccine should be given to between 20 and 100 healthy volunteers, all of whom would give informed consent.[F8] Members of the military as well as medical workers on the front lines of the Ebola outbreak are the most likely participants, Link said. Before the trial can go forward, it needs FDA approval, which he expects to come quickly: “I have never seen the FDA so supportive,” he said. FDA spokeswoman Erica Jefferson said she could not confirm that the agency is in discussions with NewLink. “The FDA is generally not allowed to disclose information about any medical product under development,” she added. At least one site for the human safety trial will be in the United States, Link said. But the possibility of conducting a trial in Africa recruiting medical workers has also been raised in frequent conversations between his company and the CDC, WHO and other agencies. Recruiting volunteers from the U.S. military is also “a distinct possibility,” Link said, given the DTRA support and Walter Reed discussions: “From what I’m hearing, I’m not worried about finding volunteers.” The NewLink vaccine uses an attenuated or weakened virus, a pathogen found in livestock called vesicular stomatitis virus (VSV). One of the virus’s genes is replaced by a gene from the Ebola virus. The Ebola gene makes a harmless protein that sits on the virus’s outer coat. The idea is that after[F9] the VSV is injected, the body’s immune system will recognize the Ebola protein as foreign and begin making antibodies that destroy Ebola viruses, all of which have the protein. Link is confident in the vaccine’s safety and efficacy, based on results in monkeys[F10]. Live, attenuated viruses can cause inflammation and other adverse reactions, but that may be deemed worth the risk given Ebola’s high fatality rate. “We’re here to help and do whatever we can” in the disastrous Ebola outbreak, Link said. “My team has been told to get it done tomorrow.”
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    Can instant noodles lead to heart disease, diabetes and stroke?
    Date:
    August 12, 2014
    Source:
    Baylor Scott & White Health
    Instant noodle eaters, take heed. Recent Baylor research shows that significant consumption of the convenient food product — ramen included — may increase a person’s risk for cardiometabolic syndrome, especially in women. The findings, recently published in The Journal of Nutrition, could shed new light on the risks of a worldwide dietary habit.–Because ramen consumption is relatively high among Asian populations, the research focused primarily on South Korea, which has the highest per-capita number of instant noodle consumers in the world. In recent years, South Koreans have experienced a rapid increase in health problems, specifically heart disease, and a growing number of overweight adults. Such changes could lead to increased mortality due to cardiovascular disease, as well as increased health care costs.–Baylor’s primary investigator on the study, Hyun Joon Shin, MD, is a clinical cardiology fellow at Baylor University Medical Center and a nutrition epidemiology doctoral student at Harvard School of Public Health.–“While instant noodle intake is greater in Asian communities, the association between instant noodle consumption and metabolic syndrome has not been widely studied,” Dr. Shin said. “I decided to investigate in order to uncover more distinct connections.”–Dr. Shin, who led the study on behalf of the Baylor Heart and Vascular Hospital (BHVH), found that eating instant noodles two or more times a week was associated with cardiometabolic syndrome, which raises a person’s likelihood of developing heart disease and other conditions, such as diabetes and stroke.–Dr. Shin also found that those results were more prevalent in women. He said that can likely be attributed to biological differences (such as sex hormones and metabolism) between the sexes, as well as obesity and metabolic syndrome components. In addition, men and women’s varied eating habits and differences in the accuracy of food reporting may play a role in the gender gap.–Another potential factor in the gender difference is a chemical called bisphenol A (BPA), which is used for packaging the noodles in Styrofoam containers. Studies have shown that BPA interferes with the way hormones send messages through the body, specifically estrogen.–Regardless of the gender-related findings or their causes, Dr. Shin said, the study represents the importance of understanding the foods we feed our bodies.–“This research is significant since many people are consuming instant noodles without knowing possible health risks,” Dr. Shin said. “My hope is that this study can lay a foundation for future research about the health effects of instant noodle consumption.”–Dr. Shin added that the study’s health implications could be substantial — particularly if it leads to people choosing healthier foods.
    Story Source-The above story is based on materials provided by Baylor Scott & White Health. Note: Materials may be edited for content and length.–Journal Reference-H. J. Shin, E. Cho, H.-J. Lee, T. T. Fung, E. Rimm, B. Rosner, J. E. Manson, K. Wheelan, F. B. Hu. Instant Noodle Intake and Dietary Patterns Are Associated with Distinct Cardiometabolic Risk Factors in Korea. Journal of Nutrition, 2014; 144 (8): 1247 DOI: 10.3945/jn.113.188441
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    Coming soon- Genetically edited ‘super bananas’ and other fruit?
    Date:
    August 13, 2014
    Source:
    Cell Press
     
    Genetically edited fruit[F11] could mean that genetically edited versions of “super bananas” that produce more vitamin A, and apples that don’t brown when cut, could be making an appearance on grocery shelves.-Recent advances that allow the precise editing of genomes now raise the possibility that fruit and other crops might be genetically improved [F12]without the need to introduce foreign genes, according to researchers writing in the Cell Press publication Trends in Biotechnology on August 13th.-With awareness of what makes these biotechnologies new and different, genetically edited fruits might be met with greater acceptance by society at large than genetically modified organisms (GMOs) so far have been, especially in Europe, they say. This could mean that genetically edited versions of GMOs such as “super bananas” that produce more vitamin A and apples that don’t brown when cut, among other novelties, could be making an appearance on grocery shelves.-“The simple avoidance of introducing foreign genes makes genetically edited crops more “natural” than transgenic crops obtained by inserting foreign genes[F13],” said Chidananda Nagamangala Kanchiswamy of Istituto Agrario San Michele in Italy.-For instance, changes to the characteristics of fruit might be made via small genetic tweaks designed to increase or decrease the amounts of natural ingredients that their plant cells already make[F14]. Genome editing of fruit has become possible today due to the advent of new tools — CRISPR, TALEN, and the like — and also because of the extensive and growing knowledge of fruit genomes.–So far, editing tools have not been applied to the genetic modification of fruit crops.[F15] Most transgenic fruit crop plants have been developed using a plant bacterium to introduce foreign genes, and only papaya has been commercialized in part because of stringent regulation in the European Union (EU). The researchers say that genetically edited plants, modified through the insertion, deletion, or altering of existing genes of interest, might even be deemed as nongenetically modified, depending on the interpretation [F16]of the EU commission and member state regulators.–Fruit crops are but one example of dozens of possible future applications for genetically edited organisms (GEOs), Kanchiswamy and his colleagues say. That would open the door to the development of crops with superior qualities and perhaps allow their commercialization even in countries in which GMOs have so far met with harsh criticism and controversy.–“We would like people to understand that crop breeding through biotechnology is not restricted only to GMOs,” he said. “Transfer of foreign genes was the first step to improve our crops, but GEOs will surge as a “natural” strategy to use biotechnology for a sustainable agricultural future.”-Story Source-The above story is based on materials provided by Cell Press. –Journal Reference-Chidananda Nagamangala Kanchiswamy, Daniel James Sargent, Riccardo Velasco, Massimo E. Maffei, Mickael Malnoy. Looking forward to genetically edited fruit crops. Trends in Biotechnology, August 2014 DOI: 10.1016/j.tibtech.2014.07.003
     
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    Brown fat protects against diabetes, obesity in humans
    Date:
    July 23, 2014
    Source:
    University of Texas Medical Branch at Galveston
    Researchers at the University of Texas Medical Branch at Galveston have shown for the first time that people with higher levels of brown fat, or brown adipose tissue, in their bodies have better blood sugar control, higher insulin sensitivity and a better metabolism for burning fat stores.–Their findings suggest that, because of the brown fat’s ability to better regulate blood sugar, this could be a potential medical weapon against diabetes.—-“We showed that exposure to mild cold raised whole body energy expenditure, increased glucose removal from the circulation and improved insulin sensitivity in men who have significant amounts of brown adipose tissue depots,” stated UTMB’s Labros Sidossis, professor of Internal Medicine, Division of Geriatric Medicine. “These results support the notion that brown adipose tissue may function as an anti-obesity and anti-diabetic tissue in humans.”—People have two types of fat tissue in their bodies: the widely reviled white fat tissue and the less familiar brown fat tissue. One of the many ill health effects of excess white fat tissue is decreased insulin sensitivity, which is a major contributor to diabetes. On the other hand, brown fat has several healthy qualities, including protection against obesity and diabetes.—In their new study appearing in the journal Diabetes, Sidossis and his colleagues compared otherwise similar healthy men with either high or low levels of brown fat tissue on their resting energy expenditure, glucose usage and insulin sensitivity. These men were placed in either normal temperature conditions or were exposed to mildly cold temperatures for five to eight hours.–Throughout the cold or regular temperature exposure period, the team conducted comprehensive analyses of various bodily samples. They collected blood and breath samples to observe changes in glucose and insulin concentrations, hormone changes, whole body oxygen consumption and carbon dioxide production rates. They also aspirated brown and white fat tissue samples to analyze differences in cellular energy production and gene expression.—“In this study we show that, when activated via mild cold exposure, brown adipose tissue can increase energy expenditure and burn calories. This is good news for overweight and obese people,” stated Sidossis. “Of even greater clinical significance may be the finding that brown fat can help the body regulate blood sugar more effectively. This is great news for people with insulin resistance and diabetes and suggests that brown fat may prove to be an important anti-diabetic tissue.”
    Story Source:
    The above story is based on materials provided by University of Texas Medical Branch at Galveston. Note: Materials may be edited for content and length.-Journal Reference-Maria Chondronikola, Elena Volpi, Elisabet Børsheim, Craig Porter, Palam Annamalai, Sven Enerbäck, Martin E. Lidell, Manish K. Saraf, Sebastien M. Labbe, Nicholas M. Hurren, Christina Yfanti, Tony Chao, Clark R. Andersen, Fernardo Cesani, Hal Hawkins, and Labros S. Sidossis. Brown Adipose Tissue Improves Whole Body Glucose Homeostasis and Insulin Sensitivity in Humans. Diabetes, July 2014 DOI: 10.2337/db14-0746 1939-327X
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