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    Atrazine—the turning point in Sexuality
    The herbicide atrazine is one of the most commonly applied pesticides in the world. As a result, atrazine is the most commonly detected pesticide contaminant of ground, surface, and drinking water. Atrazine is also a potent endocrine disruptor that is active at low, ecologically relevant concentrations. Previous studies showed that atrazine adversely affects amphibian larval development. The present study demonstrates the reproductive consequences of atrazine exposure in adult amphibians. Atrazine-exposed males were both demasculinized (chemically castrated) and completely feminized as adults[F1]. Ten percent of the exposed genetic males developed into functional females that copulated with unexposed males and produced viable eggs. Atrazine-exposed males suffered from depressed testosterone, decreased breeding gland size, demasculinized/feminized laryngeal development, suppressed mating behavior, reduced spermatogenesis, and decreased fertility. These data are consistent with effects of atrazine observed in other vertebrate classes. The present findings exemplify the role that atrazine and other endocrine-disrupting pesticides likely play in global amphibian declines.
    amphibian decline
    endocrine disruption
    sex reversal
    Atrazine is one of the most widely used pesticides in the world. Approximately 80 million pounds are applied annually in the United States alone, and atrazine is the most common pesticide contaminant of ground and surface water (1). Atrazine can be transported more than 1,000 km from the point of application via rainfall and, as a result, contaminates otherwise pristine habitats, even in remote areas where it is not used (2, 3). In fact, more than a half million pounds of atrazine are precipitated in rainfall each year in the United States (2).[F2]
    In addition to its persistence, mobility, and widespread contamination of water, atrazine is also a concern because several studies have shown that atrazine is a potent endocrine disruptor active in the ppb (parts per billion) range in fish (4, 5), amphibians (6–12), reptiles, and human cell lines (5, 13–15), and at higher doses (ppm) in reptiles (16–18), birds (19), and laboratory rodents (20–28). Atrazine seems to be most potent in amphibians, where it is active at levels as low as 0.1 ppb (6–10). Although a few studies suggest that atrazine has no effect on amphibians under certain laboratory conditions (29, 30), in other studies, atrazine reduces testicular volume; reduces germ cell and Sertoli cell numbers (11); induces hermaphroditism [F3](6, 8, 10); reduces testosterone (10); and induces testicular oogenesis [F4](7–9, 31). Furthermore, atrazine contamination is associated with demasculinization and feminization of amphibians in agricultural areas where atrazine is used (32) and directly correlated with atrazine contamination in the wild [F5](7, 9, 33, 34).
    Despite the wealth of data from larvae and newly metamorphosed amphibians, the ultimate impacts of atrazine’s developmental effects on reproductive function and fitness at sexual maturity, which relate more closely to population level effects and amphibian declines, have been unexplored. In the present study, we examined the long-term effects of atrazine exposure on reproductive development and function in an all-male population of African clawed frogs (Xenopus laevis), generated by crossing ZZ females (sex-reversed genetic males) to ZZ males (SI Materials and Methods). The advantage of using this population is that 100% of the animals tested were genetic males. As a result, all hermaphrodites and females observed are ensured to be genetic males that have been altered by endocrine disruption. We examined sex ratios, testosterone levels, sexual dimorphism, reproductive behaviors, and fertility in males exposed to 2.5 ppb atrazine throughout the larval period and for up to 3 years after metamorphosis– Results
    All of the control animals reared to sexual maturity (n = 40) were males, on the basis of external morphology, whereas only 90% of the atrazine-treated animals (36 of 40) appeared male at sexual maturity (on the basis of the presence of keratinized nuptial pads on the forearms and the absence of cloacal labia). The other 10% of atrazine-exposed animals (n = 4) lacked visible nuptial pads on the forearms and had protruding cloacal labia, typical of females (Fig. 1). Upon dissection of two of the apparent females and laparotomy in another two, we confirmed that animals with cloacal labia were indeed females from the present study, on the basis of the presence of ovaries (Fig. 1F). To date, two atrazine-induced females have been maintained, mated with control males (Fig. 1G), and produced viable eggs (Fig. 1H). The resulting larvae were all male when raised to metamorphosis and sampled (n = 100), confirming that atrazine-induced females were, in fact, chromosomal males. Furthermore, atrazine-induced females lacked the DM-W further confirming that these atrazine-induced females were indeed chromosomal males [F6]–These ZZ females expressed gonadal aromatase[F7], as did true ZW females (n = 4, from our stock colony), but ZZ males (n = 8, control or treated)– Atrazine-induced females expressed aromatase in their gonads. (Top) DMRT-1 and DM-W genes from a representative control and an atrazine-exposed adult male (M) and female (F). Morphologic sex was assigned on the basis of the presence of testes (males) or ovaries (females). (Middle and Bottom) Cyp-19 aromatase expression from gonads of the same animals genotyped at Top, along with the control gene, rpL8–Atrazine decreased androgen-dependent sperm production, mating behavior, and fertility. (A and C) Largest testicular cross-sections for representative control (A) and atrazine-exposed males (C) from 2007. (B and D) Magnification of individual tubules for control (B) and atrazine-exposed (D) males. Arrowheads in B and D show outline of tubules. Control tubules are typically filled with mature spermatozoa bundles, whereas the majority of tubules in atrazine-exposed males lack mature sperm bundles and are nearly empty, with only secondary spermatocytes (SS) along the periphery of the tubule. (E) Fertility for control (Con) and atrazine-exposed (Atr) males. Pooled data from both 2007 and 2008 study are shown. *P < 0.005 (ANOVA). (F) Fertility plotted against sperm content (percentage of tubules with mature sperm bundles) for control males (black symbols) and atrazine-exposed males (red symbols) for the 2007 (circles) and the 2008 (squares) studies. Dashed lines indicate the lower limit for controls for fertility and sperm content. Sample size differs from the number of trials because no data are available from females that did not lay eggs–Previous studies showed that atrazine demasculinizes (chemically castrates) and feminizes exposed amphibian larvae, resulting in hermaphrodites (8, 10) or males with testicular oocytes (7, 9) at metamorphosis. Since our initial publications (7, 9, 10), the effects of atrazine on amphibian development and the significance of these effects to amphibian declines have been a subject of debate (30, 35, 36). Although some investigators, including Carr et al. (6), reported statistically significant effects of atrazine on gonadal morphology in X. laevis (P < 0.0003 for multiple testes and P = 0.0042 for hermaphrodites), others, using different experimental conditions and different populations of the same species, suggested that atrazine had no effect (29). Essential to this debate, however, is (i) the terminology used to describe gonadal abnormalities; (ii) the expertise and ability of other researchers to recognize abnormalities; (iii) the possibility of natural variation in sex differentiation processes between species and even between populations (or strains) within a species (37); and (iv) the long-term consequences and significance of the observed abnormalities to amphibian reproductive fitness. Here we describe complete and functional female development in genetic (ZZ) males exposed to atrazine, not the production of hermaphrodites or males with testicular oocytes. Thus, there is no confusion in the present study regarding proper terminology or proper identification. Furthermore, because we used an all genetic (ZZ) male colony and genotyped the atrazine-induced ZZ females, there is no question that atrazine completely sex-reversed genetic (ZZ) males, resulting in reproductively functional females. — The present study thoroughly examines the long-term effects of atrazine on reproductive function in amphibians. Although a single published study attempted to examine long-term reproductive effects of atrazine in amphibians (38), the authors did not report examinations of morphology. Furthermore, their examination of fertility and breeding of atrazine-exposed males was conducted after animals were injected with reproductive hormones (human chorionic gonadotropin, hCG), effectively providing “hormone replacement therapy” and reversing the effects of atrazine. The present study represents a more thorough examination of the effects of atrazine on sex hormone production, testosterone-dependent development and morphology, male reproductive behavior, and fertility– Transcription and subsequent translation of aromatase leads to estrogen production, which in turn directs differentiation of the ovary from the undifferentiated gonad. Just as exogenous estrogen results in the differentiation of ovaries in exposed genetic (ZZ) male X. laevis (45), induction of aromatase and subsequent estrogen production likely explain the complete feminization of genetic male– Furthermore, why only some males (10% in the present population) are completely feminized, whereas their siblings are merely demasculinized, remains to be explored–Furthermore, sex-reversed males (ZZ females) are only capable of producing genetic male (ZZ) offspring, so the sex ratio in exposed populations would be skewed both by the production of atrazine-induced ZZ females as well as by the fact that ZZ females can only produce ZZ (genetically male) offspring– In fact, mathematical models suggest that this very mechanism (the production of sex-reversed all male-producing animals) could drive populations to extinction
    Where is Atrazine used
    Atrazine is used on crops such as sugarcane, corn, pineapples, sorghum, and macadamia nuts, and on evergreen tree farms and for evergreen forest regrowth. It has also been used to keep weeds from growing on both highway and railroad rights-of-way- Atrazine is used specifically to control broadleaf weeds (such as milkweed, thistle, or pigweed)
    ***************************************************************************** [F8]
    Treatment of Organophosphate Exposure
    In my experience, organophosphate exposure causes more than just the neurological problems associated with anticholinesterase activity. However, the medical literature, most of the time, concentrates on that aspect of the poisoning. Particularly in chronic poisoning, the chemical dissociates within the body, resulting in metabolites of the organic component (which appear to have slightly different properties from the original at first exposure), phosphorus (which is highly toxic and corrosive in its own right) and whatever the associated radical/chemical add-on which the manufacturers add (in the Gulf War, this appears to have been fluorine which is also toxic in its own right).* With each of these component parts, there are separate sets of symptoms, separate storage sites, separate means of elimination. When the chemicals are stored in the body, they can be mobilized back into the bloodstream (in greater concentrations than at the time of absorption) as a result of exercise, heat, emotional excitement/distress, infection, massage, exposure to nutrients via dermal route near the storage sites, or due to displacement by other chemicals/drugs to which the person is exposed. Any detox must therefore be carried out with considerable care and caution, to avoid overwhelming the body’s coping mechanisms because of overload by chemicals re-emerging into the bloodstream.
    There is precious little about chronic phosphate poisoning in modern literature; however, older books do deal with phosphorus poisoning because it was common in people manufacturing matches at that time. Body fluids containing phosphorous (particularly feces, urine, and possibly semen) will burn. Phosphorus bind strongly to mucous membranes (the cause of diarrhea and watery eyes in GWI cases). The toxic effects of chemicals can be somewhat ameliorated by ingestion of calcium acetate or calcium carbonates for phosphate binding and magnesiumand iodine for flourines – it binds to phosphorous and the organics, preventing some of the caustic effects, and helping prevent re-absorption back into the system through the intestinal wall. Both phosphorus and fluorine can be bound by calcium and magnesium. A combination of calcium citrate and magnesium oxide, in approximate physiological balance, plus potassium citrate or chloride, can be taken as needed throughout the day as symptoms are noted in the muscles, head, extremities.
    What you will need is
    Sunflower Lecithin-calcium in some form ( acetate-carbonate-chloride) magnesium( citrate-malate-chloride any one of these) potassium (chloride or citrate either one) copper –selenium and zinc mixed using 1 gram increments with the minerals ( with the exception of selenium use 1 mg( milligram) and mix 2 oz of the liquid sunflower lecithin or add ( sunflower lecithin) powder ( egg yolk lecithin would be another alternative ) never use the soy lecithin- mix them all til they are completely saturated into the SFL—this will form a liposome—and then use 1 tsp several times a day—this will flush out the glyphosates and may cause a diarrhea effect –due to the flushing and restore the colon as well with probiotic or fermented foods
    Because the organophosphates diminish absorption of nutrients while increasing the requirement for them, both this preparation, plus the calcium/magnesium and potassium mentioned above, can be dissolved in the mouth – absorbing through the mucous membranes of the mouth[F9]. Individual supplements can be taken as desired –, copper (which phosphorus binds to), zinc (balances copper), Vitamin C is also useful,– both from The corrosive effect of the phosphorus, and from the storage of fluoride which makes teeth and bones brittle. Dissolved Green Multi dissolves through the skin, too. –All fluorine exposure should be avoided – which may be difficult considering water supplies are being increasingly fluoridated. Fluoride absorbs through the skin as well as via mouth, making hot tubs and swimming pools places to add to the body’s already excessive fluoride.
    The old standby for restoring damaged myelin sheaths around nerves – lecithin – contains a whopping amount of phosphorus. However, some of it may have to be taken in order to restore neurological integrity or prevent further damage. Taking choline and inositol separately via supplement appears to dislodge stored chemicals from brain and other neurological tissue all at once, creating some rather bizarre and unsettling effectsDuring the process of detox and mending, the body’s detox and eliminatory systems need to be supported.. It needs to be noted that clothing contaminated with organophosphates cannot be washed clean, and trying to wash it with other items of clothing results in the contamination of all of them. Storage in drawers and closets will also result in contamination not only of other clothing items in the same place, but also contaminate walls, floors of closets, and the wood in the chests of drawers. Contaminated walls can be washed down first with organic soap and then immediately with a slurry of lime. This does not eliminate the chemical from the walls entirely, but creates a film which can bind to at least some of the chemicals as they out-gas. It will have to be repeated. While performing such an operation, vigorous fresh-air ventilation is essential. Protective clothing and face covering (disposable) would be helpful. Even with these precautions, there is no guarantee that such cleaning won’t expose the person doing the cleaning to enough chemicals to cause discomfort or a few days of symptoms.
    Phosphorus: Henniger, Gordon R., Chapter 4, Drug and Chemical Injury – Environmental Pathology, pp. 146-245 in Kissane, John M., ed., Anderson’s Pathology – 9th ed. (St. Louis, C.V. Mosby Co. 1990)
    Merck Manual – 13th ed., p. 1977
    McGuigan, Hugh Alister, Applied Pharmacology, pp. 743-746, (St. Louis, C.V. Mosby Co. 1940)
    Fluorine: Smith, Frank A., Fluroide Toxicity, pp. 277-283 in Corn, Morton, editor, Handbook of Hazardous Materials (San Diego, Academic Press 1993)
    Hennigar, Gordon R., Chapter 4, Drug and Chemical Injury – Environmental Pathology, pp. 146-245 in Kissane, John M., ed., Anderson’s Pathology – 9th ed. (St. Louis, C.V. Mosby Co. 1990)
    Merck Manual – 13th ed., p. 1970
    McGuigan, Hugh Alister, Applied Pharamcology (St. Louis, C.V. Mosby 1940), p. 184
    Organophosphates: Hennigar, Chapter (same as above), p. 226
    Berkow, Robert, ed., The Merck Manual of Diagnosis and Therapy – 13th ed. (Rahway, NJ, Merck, Sharp & Dohme Research Laboratories 1977), p. 1482.
    Dreisbach, Robt. H. and Wm. O. Robertson, Handbook of Poisoning: Prevention, Diagnosis and Treatment (Appleton & Lange, Norwalk, CT 1987) pp. 110-118.
    Scientists Bioengineer First Artificial Animal Limb
    Rat forelimb designed and grown in lab first step toward producing parts for transplant, researchers say
    By Margaret Farley Steele
    Thursday, June 4, 2015
    THURSDAY, June 4, 2015 (HealthDay News) — Researchers say they have taken the first steps in creating artificial replacement limbs that could be used for transplantation in the future.-Massachusetts General Hospital scientists have developed bioartificial rat forelimbs with functioning vascular and muscle tissue. (The term “bioartificial” means using biomaterials and cells to make replacement organs or limbs.) [F10]The scientists believe this process could one day work in primates — including humans — as well.-The researchers reported their findings online recently in the journal Biomaterials.-Although advances in prosthetic technology have helped the more than 1.5 million U.S. residents who are missing limbs, the artificial devices have functional and aesthetic drawbacks, the study authors noted. And transplants with donor hands, developed over the past two decades, expose recipients to risky immunosuppressive therapy for life, the researchers said.-“The composite nature of our limbs makes building a functional biological replacement particularly challenging,” said study senior author Dr. Harald Ott. -“Limbs contain muscles, bone, cartilage, blood vessels, tendons, ligaments and nerves — each of which has to be rebuilt and requires a specific supporting structure called the matrix. We have shown that we can maintain the matrix of all of these tissues in their natural relationships to each other, that we can culture the entire construct over prolonged periods of time, and that we can repopulate the vascular system and musculature,” Ott said in a hospital news release.-Ott and his colleagues explained that a recipient can provide the specific cells needed to regenerate all of the tissues that make up a limb. “What has been missing is the matrix or scaffold on which cells could grow into the appropriate tissues,” they said in the news release. -In Ott’s previous research, living cells were stripped from a donor organ with a detergent solution. The remaining matrix was repopulated with specific cells appropriate to the particular organ.-For the new study, he and his colleagues used this “decellularization” technique to tackle the complexities of a bioartificial limb, while growing muscle and vascular cells in culture. -According to the news release, the resulting limb is complete with vascular cells along blood vessel walls and appropriate fibers of muscle cells.-When transplanted into animals, blood circulated through the vascular system, and electrical stimulation of muscles flexed the wrists and digits of the animals’ paws, the researchers said. -Although animal research isn’t necessarily applicable to humans, the researchers said they also successfully decellularized baboon forearms to show the feasibility of working on a human scale.-One of the upcoming challenges, Ott said, is regrowing nerves within a limb graft and reintegrating them into a recipient’s nervous system. “Additional next steps will be replicating our success in muscle regeneration with human cells and expanding that to other tissue types, such as bone, cartilage and connective tissue,” he said.-SOURCE: Massachusetts General Hospital, news release, June 2, 2015
    Blood pressure medications can lead to increased risk of stroke
    Date:May 29, 2015
    Source:University of Alabama at Birmingham
    Summary:The importance of preventing hypertension is reinforced by a study showing anti-hypertension medicines can increase stroke risk by 248 percent, according to new research. — University of Alabama at Birmingham. “Blood pressure medications can lead to increased risk of stroke.” ScienceDaily. ScienceDaily, 29 May 2015. <>.–Untreated high blood pressure, or hypertension, wreaks havoc on the body, leading to heart disease and stroke. New research from the University of Alabama at Birmingham published in the journal Stroke shows that, although HBP medications are beneficial, it is as risky to wait for the condition to develop and then treat it to a controlled level.–A cohort of 26,785 black and white participants ages 45-plus from the Reasons for Geographic and Racial Differences in Stroke (REGARDS) study were followed for 6.3 years by a research team led by George Howard, Dr.P.H., a professor in the Department of Biostatistics in the UAB School of Public Health. At baseline, 12,327 participants were successfully treated hypertensives, meaning their HBP treatment had their systolic blood pressure < 140 mm HG, the goal level set by the American Heart Association, and 4,090 unsuccessfully treated hypertensives.–At the conclusion of the follow-up period, more than 820 participants had experienced a stroke. -The harder hypertension is to control, the higher the risk for stroke, even if the treatment is successful. Howard says the risk of stroke went up 33 percent with each blood pressure medicine required to treat blood pressure to goal. Compared to people with systolic blood pressure below 120 mmHg without treatment, hypertensive individuals on three or more blood pressure medications had a stroke risk of 2.5 times higher.-“You’re in as much trouble by the time you are on three medications that achieve excellent control as you are when you have hypertension and it is untreated, which is amazing,” [F11]Howard said. “We want to raise the issue that, despite great advances in a pharmaceutical approach, relying solely on this approach is going to come at a dear price of people’s lives.”-The way to curb the problem, Howard says, is to prevent hypertension in the first place. There are a number of proven approaches to prevent or greatly delay the development of hypertension including: 1) taking part in moderate physical activity, 2) keeping weight in normal rages, 3) eating a diet rich in fruits, vegetables and Full fat dairy products and reduced in margarines-soybean oils-vegetable oils and canola and sugars-grains-breads-pastas-rice-soy and corn-eliminate the microwave.–“It’s everything we know we should be doing,” Howard said. “And over the past 14 years, stroke deaths are down 42 percent, likely because of this general shift of everybody in the population working toward having lower blood pressures.”–Howard suggests that some future efforts to prevent the development of hypertension will have to be made through policy changes, such as increasing activity and limiting body weight require individual commitment.-“We need to keep the pressure to keep good things happening from a policy standpoint,” Howard said. “Also, as individuals, we need to take the right actions for our health. Individuals and society need to work together to keep people from becoming hypertensive.”-Study co-authors include Virginia J. Howard, Ph.D., professor in the Department of Epidemiology, Paul Muntner, Ph.D., professor and vice chair in the Department of Epidemiology, and Suzanne Oparil, M.D., professor in the Division of Cardiovascular Disease.–Story Source-The above story is based on materials provided by University of Alabama at Birmingham. The original article was written by Nicole Wyatt. Note: Materials may be edited for content and length.
    Life-prolonging protein could inhibit aging diseases
    Date:May 28, 2015
    Source:Waseda University
    Summary:Researchers have found a molecule that plays a key link between dietary restriction and longevity in mammals. This discovery may lead to the development of new therapies to inhibit age-related diseases. Waseda University. “Life-prolonging protein could inhibit aging diseases.”;.–Researchers have found a molecule that plays a key link between dietary restriction and longevity in mammals. This discovery may lead to the development of new therapies to inhibit age-related diseases.–Studies have shown that moderate dietary restriction can increase the lifespan of many organisms, including mammals, while inhibiting age-related diseases such as cancer and Alzheimer’s.-A team of researchers from Japan and Korea set out to understand how this happens. They discovered that a protein, called neuropeptide Y (NPY), which transmits nerve impulses in the brain and autonomic nervous system, is an essential link between dietary restriction and longevity in mice.-The researchers found that dietary restriction did not increase the lifespan of mice devoid of NPY to the same degree that it did in wild mice that had this protein. Dietary restriction also did not have the same protective effect against tumour formation and stress that it did in wild mice.-On the other hand, other adaptive processes that are activated during dietary restriction, such as changes in energy metabolism, were not affected by the absence of neuropeptide Y.–What’s more, other researchers have found that an over-expression of the NPY gene increased the lifespan of rats that were not exposed to dietary restriction.–The Japan-Korea team’s research, published in Scientific Reports, suggests that activating certain organs or tissues — such as the liver, heart, other muscles and blood vessels — with NPY could play a role in preventing age-related disorders, including cancer and Alzheimer’s.–The researchers are currently engaged in joint exploratory research with pharmaceutical and food companies to develop substances that increase the expression of NPY as a deterrent to age-related diseases. Neuropeptide Y molecule. This neurotransmitter peptide plays an important role in food intake.-Story Source-The above story is based on materials provided by Waseda University. Journal Reference- Takuya Chiba, Yukari Tamashiro, Daeui Park, Tatsuya Kusudo, Ryoko Fujie, Toshimitsu Komatsu, Sang Eun Kim, Seongjoon Park, Hiroko Hayashi, Ryoichi Mori, Hitoshi Yamashita, Hae Young Chung, Isao Shimokawa. A key role for neuropeptide Y in lifespan extension and cancer suppression via dietary restriction. Scientific Reports, 2014; 4 DOI: 10.1038/srep04517
    Mesoporous particles for the development of drug delivery system safe to human bodies
    National Institute for Materials Science. “Mesoporous particles for the development of drug delivery system safe to human bodies.” ScienceDaily. ScienceDaily, 20 May 2015. <>.
    Date:May 20, 2015
    Source:National Institute for Materials Science
    Summary:Scientists have succeeded in developing porous particles (mesoporous particles) consisting solely of phospholipids, a biological component, that are suitable for use as a drug delivery system–A research group led by MANA Scientist Kohsaku Kawakami, postdoctoral researcher Shaoling Zhang and MANA Principal Investigator Katsuhiko Ariga, at the International Center for Materials Nanoarchitectonics (MANA), NIMS (Sukekatsu Ushioda, President), succeeded in developing porous particles (mesoporous particles) consisting solely of phospholipids, a biological component, that are suitable for use as a drug delivery system[F12]. This study had been published in the Journal of Physical Chemistry C on 16 March, 2015.–Mesoporous materials are a type of material capable of serving as a drug delivery system[F13]. In conventional studies, hard materials such as silica and carbon materials have been used for such purposes, posing safety concerns to patients. The mesoporous material developed in this study consists exclusively of biologically-derived materials and is therefore expected to be very safe for humans.–Acquisition of official approval is one of the hurdles in the development of materials for use as a drug delivery system. To develop a certified pharmaceutical product, it is necessary to demonstrate the safety of the additive to be used before investigating the safety of the product itself. For this reason, pharmaceutical companies tend to avoid using new additives, which had been slowing the development of new drug carriers. However, the phospholipids examined in this study have already been used as emulsions and liposomes, and thus are not regarded as new additives. This fact is a great advantage of this material in view of commercialization.–This material comprises highly uniform mesoporous particles with diameters ranging between 5 and 20 μm[F14], depending on their composition. It is a very lightweight material with a bulk density of about 0.02 g/cm3, from which an aerodynamic diameter of 1 to 3 μm is calculated. These are ideal features for this material to be used as a powder inhalation carrier.–Since this material consists of lipid bilayer membranes that are similar to biological membranes, it possesses the characteristics of both mesoporous particles and liposomes. For example, it can be used with both hydrophobic and hydrophilic drugs. Hydrophobic drugs can be embedded in a lipid bilayer membrane, and hydrophilic drugs can be inserted into hydrophilic regions between lipid bilayer membranes. Furthermore, as it is also feasible for the material to hold drugs in its mesopores, the material is capable of carrying drugs with various physical properties. Since phospholipids can be easily modified, it is conceivable that various kinds of surface modifications can be applied to the material.–This material is suited for industrial production as it can be easily prepared through freeze-drying. And it is expected to be useful as a drug carrier assuming any administration route and as a cosmetic ingredient. The unique shape of the particles also may add value to the commercial product.–Story Source-The above story is based on materials provided by National Institute for Materials Science. Journal Reference-Shaoling Zhang, Kohsaku Kawakami, Lok Kumar Shrestha, Gladstone Christopher Jayakumar, Jonathan P. Hill, Katsuhiko Ariga. Totally Phospholipidic Mesoporous Particles. The Journal of Physical Chemistry C, 2015; 119 (13): 7255 DOI: 10.1021/acs.jpcc.5b00159
    [F1]Basically a homosexual effect in an amphibian
    [F2]And that is just in the USA not to mention Canada or Europe or Asia-Africa–etc
    [F3]Hermaphrodite—but male and female
    [F5]Sexual Alteration
    [F7]Male Hormone
    [F8]This may also be true with other species when the endocrine system is off or altered it could carry over into the next generation
    [F9]Another method would be to mix in a yogurt or kefir utilizing the bacteria to break down these elements so that they are more useable to the system
    [F10]Potential Nano
    [F11]So taking the meds do not work
    [F12]This would be similar toa liposomal delivery method using a phosphorous based fat ( egg-sunflower lecithin-egg yolk lecithin) to transport neutrients through the body and cells
    [F13]Or vitamins or herbs or minerals
    [F14]Micron or micrometer which is safe to utilize
    Top A
    Script of the Show June 13 2015
    Caffeine and gold to kill cancer cells
    Low glycemic index diet reduces symptoms of autism in mice
    Catalysts safely and effectively remove estrogenic compounds from wastewater
    Caffeine and gold to kill cancer cells
    March 3rd, 2014 | Author: Daniel
    The side effects of consuming too much caffeine (e.g., agitation, rapid heartbeat and difficulty sleeping) are well known, but recent research has shown that stimulant also has its good side. You can kill cancer cells. Now, an international team of scientists has found that the combination of caffeine-based compound and a small amount of gold could someday be used as an anticancer agent.–Caffeine and certain compounds based on it have recently been in the spotlight as potential anticancer treatments. But it is clear that drinking liters of coffee, caffeinated sodas and energy drinks, is not the solution, and the caffeine in these drinks at the levels needed to kill cancer cells, they also begin to have negative effects on healthy. Meanwhile, gold can also kill cancer cells, but, like caffeine, can damage. Given all this, the team of Michel Picquet, Institute of Molecular Chemistry, University of Burgundy in Dijon, France, and Angela Casini, Institute of Pharmacological Research of the University of Groningen in the Netherlands, decided to put the two together in certain configurations, to see if the new compounds based on caffeine and gold can act selectively against cancer cells, preventing growth without damaging the other.-For this purpose a series of seven prepared new compounds in the laboratory and studied. The scientists found that, in certain concentrations, one of the series compounds selectively killed human ovarian cancer cells without harming healthy cells. Furthermore, the compound acted specifically against architecture of DNA that is associated with cancer.-In the research and development have also participated Loic Stefan, Marc Pirrotta, David Monchaud, Ewen Bodio, Philippe Richard and Pierre Le Gendre, Institute of Molecular Chemistry, University of Burgundy in Dijon, France, Elena Warmerdam, Marina H. de Jager and Geny MM Groothuis, Institute of Pharmacological Research of the University of Groningen in the Netherlands, and Benoit Bertrand of both institutes.
    Low glycemic index diet reduces symptoms of autism in mice
    Date-June 9, 2015
    Source-Salk Institute
    Summary-The number of people diagnosed with autism — a spectrum of disorders characterized by social avoidance, repetitive behaviors and difficulty communicating — has risen dramatically over the past two decades for reasons that are unclear. A diet recommended for diabetics ameliorated signs of autism in mice, researchers have found. Although preliminary and not yet tested in humans, the findings might offer clues to understanding one potential cause of autism. —The brains of mice fed a high glycemic index diet have greater numbers of activated immune cells (shown in red and green) called microglia.—Bread, cereal[F1] and other sugary processed foods cause rapid spikes and subsequent crashes in blood sugar. In contrast, diets made up of vegetables, fruits are healthier, in part because they take longer to digest and keep us more even-keeled.—New research in a mouse model of autism showed that such low glycemic index diets, similar to the plans that people with diabetes follow to keep their blood sugar in check, reduced symptoms of the disorder in mice. Although preliminary and not yet tested in humans, the findings, published June 9 in the journal Molecular Psychiatry, might offer clues to understanding one potential cause of autism.–The number of people diagnosed with autism — a spectrum of disorders characterized by social avoidance, repetitive behaviors and difficulty communicating — has risen dramatically over the past two decades for reasons that are unclear.–More people may be diagnosed due to a broader definition of autism and better efforts in diagnosis, but a true increase in the disorder cannot be ruled out, according to the U.S. Centers for Disease Control. Lifestyle change is one potential factor out of many possible causes of autism.–‘One thing that’s driving a lot of general physiological changes in people is changes in the diet,’ says the study’s corresponding author Pamela Maher, a senior staff scientist in the laboratory of professor David Schubert at the Salk Institute for Biological Studies.–In the new study, the Salk scientists used a mouse model of autism — an inbred strain of mouse previously found to display autism-like symptoms — to ask whether lowering the level of dicarbonyl methylglyoxal (a common byproduct of sugar metabolism) could alleviate symptoms of autism in the animals.–The scientists fed pregnant mice either the high or low glycemic index diet and kept their offspring on the same diet after birth and weaning, because their brains are still forming crucial connections.–The researchers then used a battery of behavioral and biochemical tests to study the mice after weaning. The two groups of animals consumed the same number of calories and were identical in weight. But mice that ate a high-glycemic index diet showed all of the expected behavioral symptoms of autism[F2]. Their social interactions were impaired, they repeated actions that served no apparent purpose, and they groomed extensively.
    The mouse models of autism on a normal lab diet (with a medium glycemic index) are already known to generate fewer new neurons, and some of their existing cells and neuronal connections are abnormal compared with those of normal mice.–Intriguingly, in the new study, the brains of mice modeling autism that were fed the high-glycemic index diet had drastically less doublecortin, a protein indicator of newly developing neurons, compared to predisposed mice on the low-glycemic index diet. The deficiency was especially obvious in a part of the brain that controls memory.–In addition, the brains of the high-glycemic index diet mice appeared to have greater numbers of activated microglia, the resident immune cells of the brain. Their brains also expressed more genes associated with inflammation, compared to the mice fed the low-glycemic index diet.—Other studies of human mothers and their children with autism have implicated the activation of the immune system. For the most part, these studies have focused on infection, which causes a bout of inflammation — as opposed to a high-glycemic index diet, which causes chronic, low-level inflammation, Maher says.–The new study found that the diet might directly influence the ecosystem of bacteria in the gut. More complex starches are broken down by bacteria that live in the lower part of the gut, the large intestine. The group saw some evidence of that in the blood, detecting metabolites that could only have come from the gut in larger amounts in the animals fed the high-glycemic index diet.–‘We were really surprised when we found molecules in the blood that others had reported could only be generated by gut bacteria,’ Maher says. ‘There were big differences in some of these compounds between the two diets.’–The group plans to analyze the gut bacteria, and its potential link with features of autism, more directly. They also hope to better understand the role of inflammation in the ability to generate new neurons.–Lastly, they plan to vary the timing of exposure to the various diets in the mouse model of autism, by, for example, giving pregnant mice a high-glycemic index diet and then keeping their pups on a normal diet.–Story Source-The above story is based on materials provided by Salk Institute. -Journal Reference-A Currais, C Farrokhi, R Dargusch, M Goujon-Svrzic, P Maher. Dietary glycemic index modulates the behavioral and biochemical abnormalities associated with autism spectrum disorder. Molecular Psychiatry, 2015; DOI: 10.1038/mp.2015.64
    Catalysts safely and effectively remove estrogenic compounds from wastewater
    Date-June 12, 2015
    Source-Carnegie Mellon University
    Summary-Catalysts effectively and safely remove a potent and dangerous endocrine disruptor from wastewater. Scientists have demonstrated that the catalysts could be a viable option for large-scale water treatment.–Catalysts created by Carnegie Mellon University chemist Terrence J. Collins effectively and safely remove a potent and dangerous endocrine disruptor from wastewater.–In a paper published in Scientific Reports, Collins’ research team and collaborators led by Brunel University London’s Susan Jobling and Rak Kanda demonstrate that the catalysts could be a viable option for large-scale water treatment.–As pharmaceutical use has skyrocketed, especially in first-world countries, the amount of drugs released into the water system through wastewater has dramatically increased. Medications designed to disrupt the endocrine system, such as birth control pills and some breast and prostate cancer drugs, can be found in close to 25 percent of the world’s streams, rivers and lakes. Studies have shown that these compounds have an adverse effect on the health of wildlife.–In many cases, researchers are finding that male fish in these polluted water sources undergo a process called feminization, which is an indicator that estrogenic contaminants are present in the water[F3]. Prolonged exposure to these female hormones can cause males to develop eggs in their testes and leads to the decline of fish populations.–“Unfortunately, some synthetic chemicals, including some everyday chemicals, are powerful endocrine disruptors and they often turn up as contaminants in water. These chemicals, called micropollutants,[F4] can be bioactive at low environmentally relevant concentrations and are typically tough to break down,” said Collins, the Teresa Heinz Professor of Green Chemistry at Carnegie Mellon. “We need to get these micropollutants out of our water systems. Fish are indicators of what can happen when hormone control systems get hijacked by synthetic chemicals. We humans are also animals with endocrine systems, after all.”–When a person takes a drug, that drug travels through their body and what isn’t absorbed or broken down is excreted as waste[F5]. Conventional wastewater treatment systems are unable to fully remove many of the harmful chemicals found in today’s pharmaceuticals, pesticides and other products[F6]. Advanced processes installed at the end of wastewater treatment plants, especially those that use ozone or activated carbon, have been shown to be effective options for reducing micropollutants,[F7] but the high financial and energy costs of incorporating these have limited their adoption.–Collins has developed a group of catalysts called TAML activators that offer an alternative treatment option. TAMLs are small molecules that mimic oxidizing enzymes.[F8] When combined with hydrogen peroxide, TAML activators very effectively break down harmful chemicals in water. To test the effectiveness and safety of these catalysts, Collins teamed up with the Brunel research team, who are world-class experts in aquatic toxicity and wastewater treatment. In the current paper, the group demonstrates the efficacy and safety of TAML activators via a series of experiments. First, they showed that TAMLs were able to degrade, in pure water, 17alpha-ethinylestradiol (EE2), a synthetic estrogen found in oral contraceptives and a major cause of fish feminization. They then isolated the early intermediate compounds created as TAMLs degrade EE2, and found that several of these were estrogenic and harmful, too. But, using chemical analysis, the researchers showed that the TAML process was able to effectively degrade these intermediate compounds.–The research group also applied TAML activators to samples of water processed by municipal wastewater plants from the U.K. They found that the TAMLs were able to break down EE2 and other estrogenic compounds and micropollutants in the water.–The researchers were then able to demonstrate in the lab that water treated with TAMLs was not harmful to fish. They exposed male fathead minnows, a common freshwater fish found in many inland waterways, to water containing EE2. The exposure to EE2 caused a well-known effect — the fish began to feminize. After they used TAMLs to remove EE2 from the water, the amount of vitellogenin, a female egg yolk protein, found in the minnows significantly decreased, signaling a dramatic reduction in feminization. Additionally, the fish did not have any detectable adverse effects from being exposed to the tiny traces of TAMLs in the water.–The researchers plan to test TAMLs against ozone and activated carbon treatment systems. They have shown that TAMLs will be at least as effective, and anticipate that the TAML process will come at a much lower cost. Collins estimates that a kilogram of catalyst could treat tens of thousands of tons of wastewater.–Story Source-The above story is based on materials provided by Carnegie Mellon University. Journal Reference-Matthew R. Mills, Karla Arias-Salazar, Alice Baynes, Longzhu Q. Shen, John Churchley, Nicola Beresford, Chakicherla Gayathri, Roberto R. Gil, Rakesh Kanda, Susan Jobling & Terrence J. Collins. Removal of ecotoxicity of 17α-ethinylestradiol using TAML/peroxide water treatment. Scientific Reports, 2015 DOI: 10.1038/srep10511
    1. I, _______________________________, being over the age of 18, having firsthand knowledge of the facts contained herein, and being competent to testify to these matters, declare as follows: 2. Title 21 of the U.S. Code, Section 321 of The Food, Drug, and Cosmetic Act, purports to be “law” expressly applying to People whom it describes by the phrases “man or other animals” and “animals other than man”. These phrases deem, declare and/or presume Americans to be animals in violation of fundamental principles of the Abrahamic Faiths (Judaism, Christianity and Islam) and violate the Organic Laws of the United States of America; the Declaration of Independence, the Establishment Clause and Free Exercise Clause of the First Amendment to the Constitution for the United States of America, as well as Article 18 of the Universal Declaration of Human Rights and statutes found at 18 U.S.C. §§ 241-242. 3. As one of the People with a sincere faith in the existence of a Creator having endowed mankind with certain God-given unalienable Rights, pursuant to the Organic Laws of the United States of America and the Declaration of Independence, I am adversely affected,
    VERIFIED DECLARATION OF LIVING MAN / WOMAN ­ Pg 1 –injured and/or damaged by any law, Act of Congress, and/or conspiracy which would deem me and/or fellow members of mankind and the People of The United States of America to be “animals”. “MAN OR OTHER ANIMALS” LAWS 4. 21 U.S.C. § 321(g) defines the term “drug” in relevant part as “(A) …; and (B) articles intended for use in the diagnosis, cure, mitigation, treatment, or prevention of disease in man or other animals; and (C) articles (other than food) intended to affect the structure or any function of the body of man or other animals…” [Emphasis added]. 5. Numerous other instances of the phrases “man or other animals” and/or “animals other than man” exist in The Food, Drug and Cosmetic Act, as well as in the food and drug laws of the several states. 6. The phrases “man or other animals” and “animals other than man” mean that all men to which such food and drug laws are intended to apply are declared and/or presumed to be “animals”. 7. The declaration and/or presumption that all men are “animals” violate fundamental principles of religious freedom and God-given unalienable Rights; evades the solemn obligations of government and implicates genocide. RELIGIOUS FREEDOM 8. Regardless of one’s faith or religion, one of the most fundamental principles upon which The Declaration of Independence was written is the undeniable and irrefutable existence of all that is, indicating a Creator as written in Genesis 1:1 which states: “In the beginning, God created the heavens and the earth.” By virtue of this creative Act, the Creator, however regarded or worshipped by man, owns perfect title to that Creation. 9. A second fundamental principle is found at Genesis 1:26-29 which declares that on the sixth day, God created man, and man alone, in God’s image, and gave man dominion over all the animals. Thus man, having been created in the image of the Creator and having been given dominion OVER ALL THE ANIMALS, cannot therefore be deemed an –
    VERIFIED DECLARATION OF LIVING MAN / WOMAN ­ Pg 2 -“animal” by any law of any man. Any “man” who subjects himself to such a law or accepts the status of “animal” violates this most fundamental principle. 10. The U.S. Code defines the Organic Laws of the United States to include The Declaration of Independence, which states in part: “We HOLD these Truths to be self-evident, that all Men are CREATED equal, that they are endowed by their Creator with certain unalienable Rights…” 11. The term “hold” is a legal term with which the judiciary is well acquainted. It means to “preserve as a matter of law”. It is held ­ preserved, as a matter of law, by the very Organic Laws of the United States of America, that “all men are created” and are “endowed by their Creator” ­ with certain God-given unalienable Rights. 12. As per Acts 17:26, all men are created from the same blood. No man can be treated as an animal. As per Acts 17:27, the Creator appoints to each man the task to “seek the [Creator] in the hope that they might find Him, though He is not far from each one of us…” 13. Any de jure or de facto government, governmental agency and/or corporation that acts to degrade any man created in the Creator’s image to the status of an “animal” would trespass upon that man’s religious freedom, interfere with that man’s search for his Creator by implicitly denying the Creator’s existence, and/or seek to subject that man to an establishment of a religion such as secular humanism, naturalism, or Darwinism. 14. The unanimous Declaration of the original thirteen united States of America, as well as Article 1, Section 11 of the Constitution of The State of Washington, and the First Amendment to The Constitution of the United States of America; each include fundamental principles, guarantees and/or prohibitions that secure each man’s right of religious freedom and right to be free from the establishment of religion. These same instruments impose solemn obligations upon government to secure such rights and entitle every man to seek redress for violations by government of such obligations. 15. Any state or federal law that deems, declares, or presumes man to be an “animal” is anathema to my faith. Such laws constitute the establishment and imposition of a VERIFIED DECLARATION OF LIVING MAN / WOMAN ­ Pg 3 -religion of secular humanism, naturalism, or Darwinism upon the People of The United States of America. Any act by any state or federal government or governmental agency that directly or indirectly degrades mankind to the status of “animals” likewise constitutes the establishment of a religion of secular humanism, naturalism, or Darwinism upon the People of The United States of America. Such “man or other animals” law(s) are facially unconstitutional and unconstitutional as applied in the matter before this tribunal. 16. I hereby object to any law that declares or presumes me or my fellow man to be an “animal”. As a matter of love for my neighbors, I object on behalf of all mankind and the People of The United States of America, to any such law that degrades the People to the status of “animals”. 17. Any decision knowingly based on laws that presume and/or declare all men to be “animals” would constitute a conspiracy to violate and/or deny the God-given, unalienable Rights of the People of The United States of America to religious freedom and/or freedom from a government-imposed establishment of religion. Such a decision would further damage said People by depriving them of their standing as “sovereigns without subjects” and “joint-tenants in the sovereignty” as declared by The Supreme Court of the United States in Chisholm v State of Georgia, 2 Dall. 419 (A.D. 1793). 18. Parties to this matter may not have previously understood that The Food, Drug, and Cosmetic Act deprives the People of their God-given, unalienable Right to religious freedom and/or freedom from establishment of religion. Parties to this matter might previously have argued that they did not knowingly intend to deprive People of such rights. However, thanks to this Notice, all parties now know or have reason to know that such further action based upon laws repugnant to the Constitution would cause injury and/or damages to me and the People of The United States of America. IMPLICATIONS OF CONSPIRACY TO COMMIT GENOCIDE 19. The article entitled “Genocide” reports in part, “In 1996, Gregory Stanton, the president of Genocide Watch, presented a briefing paper called `The Eight
    VERIFIED DECLARATION OF LIVING MAN / WOMAN ­ Pg 4 Stages of Genocide’ at the United States Department of State. In it, Stanton suggests that genocide develops in eight (8) stages that are “predictable but not inexorable.” Those eight (8) stages include: 1) Classification; 2) Symbolization; 3) Dehumanization; 4) Organization; 5) Polarization; 6) Preparation; 7) Extermination; and 8) Denial. 20. Stage #3 (“Dehumanization”) is described in part as “One group denies the humanity of the other group. Members of it are equated with animals, vermin, insects or diseases.” [Emphasis added]. 21. History tells us that African-Americans were enslaved based on the most evil of premises that they were not “men” but chattel (property or “animals”). History tells us they fought the civil rights movement in large measure to be recognized as “men”. The Civil War and the deaths of 750,000 Americans followed, in part, from the horrible premise that some men were “animals”. 22. History also tells us that during World War II, Jews suffered extermination at the hands of the Nazi’s based on the most evil of premises that the Jews were “untermenschen” (less than men or “animals”). Nazis could slaughter even Jewish women and children because to them they were no more than “animals”. Millions of men and women died, the world was plunged into its most tragic war, and the nation of Germany was nearly destroyed based in part on the horrible premise that some men were “animals” 23. More recent history tells us that in April of 1994, in the African country of Rwanda, nearly a million men, women and children of the Tutsis and Hutus were hunted with machetes like animals, without any regard to their humanity. 24. Those state or federal food and drug laws which declare or presume man to be an “animal” conform to Stage Three of “The Eight Stages of Genocide”, namely “Dehumanization” and characterization as “animals”. 25. The United Nations’ Convention on the Prevention and Punishment of the Crime of Genocide, approved December 9th, A.D. 1948, declares in part, “genocide is a crime under international law” — but that crime need not include the actual deaths of any victims. For example, Article 2 of said Convention declares in part, “… genocide means
    VERIFIED DECLARATION OF LIVING MAN / WOMAN ­ Pg 5-any of the following acts committed with intent to destroy, in whole or in part, a national, ethnical, racial or religious group, as such: … Causing serious bodily or mental harm to members of the group; (c) Deliberately inflicting on the group conditions of life calculated to bring about its physical destruction in whole or in part; …” [Emphasis added]. 26. The state and federal “man or other animals” laws inflict “mental harm” and/or impose “conditions of life calculated to bring about the physical destruction in whole or in part” upon those sovereign People of The United States of America who are made in the Creator’s image (as per Genesis 1:26-29) and “endowed by their Creator with certain unalienable Rights” (as per The unanimous Declaration of the thirteen united States of America of July 4th, A.D. 1776). This injury is achieved by refusing to recognize said People’s direct relationship to the Creator by implicitly denying the existence of the Creator and by degrading the People from the status of men, whose rights flow from the Creator, to the status animals whose “rights” — if any — flow from the government. 27. Article 3 of said Convention declares, “The following acts shall be punishable: (a) Genocide; (b) Conspiracy to commit genocide; (c) Direct and public incitement to commit genocide; (d) Attempt to commit genocide; (e) Complicity in genocide.” [Emphasis added]. 28. Article 4 of said Convention declares, “Persons committing genocide or any of the other acts enumerated in article III shall be punished, whether they are constitutionally responsible rulers, public officials or private individuals.” 29. Until now, parties in this matter may have had no understanding of the genocidal implications of their contemplated actions and thus no intent to engage in genocide. However, thanks to this Notice — which I provide as an act of love for my neighbors — the parties now know or have reason to know the genocidal implications of contemplated action and/or conspiracy based on “man or other animals” laws. 30. Should said parties proceed in their contemplated actions and/or conspiracy, they do so knowing or having reason to know that that such conspiracy may be found to constitute
    VERIFIED DECLARATION OF LIVING MAN / WOMAN ­ Pg 6 an act of genocide as defined by the United Nations’ Convention on the Prevention and Punishment of the Crime of Genocide. THE POWER OF A JURY 31. John Jay was the first Chief Justice of the U.S. Supreme Court, and the first President after the American Revolution in 1789. He said: “The Jury has a right to judge both the law as well as the fact in controversy.” 32. Samuel Chase, who was a signatory to the Declaration of Independence and Associate Justice of the U.S. Supreme Court in 1796 said: “The jury has the right to determine both the law and the facts.” 33. Oliver Wendell Holmes, another U.S. Supreme Court Justice in 1902 said: “The jury has the power to bring a verdict in the teeth of both law and fact.” See Homing v. District of Columbia, 138 (1920). 34. Harlan F. Stone, 12th Chief Justice of the U.S. Supreme Court in 1941 said: “The law itself is on trial quite as much as the cause which is to be decided.” 35. Justice Stone also said: “If a juror feels that the statute involved in any criminal offence is unfair, or that it infringes upon the defendant’s natural god-given unalienable or constitutional rights, then it is his duty to affirm that the offending statute is really no law at all and that the violation of it is no crime at all, for no one is bound to obey an unjust law.” 36. The Supreme Court said in Marbury v. Madison (5US137, 176; 1803): “All laws which are repugnant to the Constitution are null and void.” 37. In U.S. v Moylan (1969), the 4th Circuit Court of Appeals said “If the jury feels the law is unjust, we recognize the undisputed power of the jury to acquit, even if its verdict is contrary to the law as given by a judge, and contrary to the evidence.” 38. Lysander Spooner said in The Right of Juries: “For more than six hundred years ­ that is, since Magna Carta, in 1215 ­ there has been no clearer principle of English or American constitutional law, than that, in criminal cases, it is not only the right and duty of juries to judge what are the facts, what is the law, and what was the moral intent of the
    VERIFIED DECLARATION OF LIVING MAN / WOMAN ­ Pg 7 accused; but that it is also their right, and their primary and paramount duty, to judge the justice of the law, and to hold all laws invalid, that are, in their opinion, unjust or oppressive, and all persons guiltless in violating, or resisting the execution of, such law.” 39. In the recent U.S. Supreme Court case Bond vs. United States, No. 09-1227 (2011), the Court said: “‘An offence created by [an unconstitutional law],’ the Court has held, is not a crime.' Ex parte Siebold, 100 U.S. 371, 376 (1880).A conviction under [such a law] is not merely erroneous, but is illegal and void, and cannot be a legal cause of imprisonment.’ Id., at 376­377. If a law is invalid as applied to the criminal defendant’s conduct, the defendant is entitled to go free … In short, a law beyond the power of Congress,' for any reason, isno law at all.’ Nigro v. United States, 276 U. S. 332, 341 (1928).” 40. Thomas Jefferson is attributed to saying “If people let the government decide what foods they eat and what medicines they take, their bodies will soon be in as sorry a state as are the souls of those who live under tyranny.” 41. The U.S. Food and Drug Administration (“FDA”), together with the U.S. Department of Justice (“DOJ”), seeks to charge, indict, or convict Mr. Daniel Smith of Project GreenLife, and others, for alleged violations of The Food Drug and Cosmetic Act. Mr. Smith could face many years in prison if convicted. However, The Food, Drug, and Cosmetic Act exceeds Congress’ enumerated powers because it violates the Establishment Clause and Free Exercise Clause of the First Amendment to Constitution for the United States of America, which states in pertinent part: “Congress shall make no law respecting an establishment of religion, or prohibiting the free exercise thereof;” 42. The Declaration of Independence states: “… that to secure these Rights, Governments are instituted among Men, deriving their just Powers from the Consent of the Governed, that whenever any Form of Government becomes destructive of these Ends, it is the Right of the People to alter or abolish it…” 43. It is the right of the jury to HOLD (preserve as a matter of law), that The Food, Drug, and Cosmetic Act by its own definitions, which deem, declare and/or presume Americans
    VERIFIED DECLARATION OF LIVING MAN / WOMAN ­ Pg 8 and all mankind to be merely “animals”, violates God-given unalienable rights guaranteed by the Constitution for the United States of America, as well as Article 18 of the United Nations’ Universal Declaration of Human Rights, which states: “Everyone has the right to freedom of thought, conscience and religion; … and freedom … in public or private, to manifest his religion or belief in teaching, practice, worship and observance. 44. It is the right of the jury to HOLD (preserve as a matter of law), that The Food, Drug, and Cosmetic Act by its own definitions, which deem, declare and/or presume Americans and all mankind to be merely “animals”, conforms to Stage (3) Three of “The Eight Stages of Genocide” wherein “man” is characterized as an “animal”. 45. In 1980, an American screenplay received eight (8) Academy Award nominations including “Best Picture”. It depicted a man being chased on the streets of London, unmasked, and cornered by an angry mob, at which point he cries out before collapsing… “I AM NOT AN ANIMAL … I AM A HUMAN BEING … I … AM … A … MAN”. This screenplay was an adaptation from the books by Sir Frederick Treves and Ashley Montagu… The Elephant Man: A Study in Human Dignity. 46. The Food, Drug, and Cosmetic Act evades all manner of human dignity by deeming, declaring and/or presuming mankind to be merely “animals”. We the People must stand up ­ for human dignity and for God-given unalienable Rights guaranteed by the Constitution for the United States of America, and to remind the Government that “Governments are instituted among Men, deriving their just Powers from the Consent of the Governed,” and “that whenever any Form of Government becomes destructive of these Ends, it is the Right of the People to alter or abolish it.” 47. Consistent with my faith, State Constitutions, and with the Organic Laws of the United States, including the Declaration of Independence and the Constitution for the United States of America and Article 18 of the Universal Declaration of Human Rights, I declare and give notice of the assured value and preservation of my God-given, unalienable Rights, my right to religious freedom, my right to be free from the establishment of religion, and that I and my fellow man, are NOT, nor ever shall be “animals”.
    48. If we are prepared to accept the label of “animal”, we will be treated like animals. The government derives its power from the consent of the governed and I, for one, do not consent. I AM NOT AN ANIMAL! 49. Based upon the foregoing, the jury should exercise its right NOT to charge, indict or convict Daniel Smith, and should effectively return an acquittal – or a NO TRUE BILL OF INDICTMENT. The jury should use its unique and unequivocal Power to ferret out real crimes committed by real criminals beginning with government agencies like the FDA who seem to be perpetually engaged in the intentional abrogation of these, our Godgiven, natural, and unalienable rights.
    I, ________________________________, hereby verify, under penalty of perjury, under the laws of the United States of America that the above statement of facts and laws is true and correct, according to the best of my current information, knowledge, and belief, so help me God, pursuant to 28 U.S.C. § 1746(1). Dated this _________ Day of _____________, 2012 A.D.
    Signed Name: _______________________________________ Printed Name: _______________________________________ Address: _________________________________________ City, State, Zip: ______________________________________
    A. Clinton Ober Ventura, CA INTRODUCTION
    From the beginning of time, except for the past few generations, humans lived their entire lives primarily in direct physical contact with the earth; therefore, it is assumed that humans throughout evolution were naturally grounded. In modern times, humans have insulated themselves from contact with the earth by wearing synthetic soled shoes and living in homes that elevate the body above the earth. Consequently, humans are no longer naturally grounded and now the body becomes charged with static electricity and radiated electric fields can now create unnatural weak electric currents with-in the body[F9]. [1] This work provides evidence that loss of natural ground allows extraneous electricity to interfere with and stress the normal bioelectrical activities of the body, which thereby interferes with natural health and sleep. Today everyone is physically stressed, their muscles are tense, back and joint pain are the norm and most do not sleep well. These conditions all relate to excess stimulation of the nervous system and/or interference of the bioelectrical communications between cells. For instance, muscles only respond to bio-electrical communications from nerves. When these communications are interfered with muscles become tense and remain tight. This leads to fatigue, skeletal problems and pain. To what extent do EMFs create abnormal electrical activity in/or on the body? In 1995 the National Institute of Environmental Health Sciences [NIEHS] and the US Department of Energy [DOE] stated that common exposure to electric and magnetic fields [EMFs] from household electrical wires now produce unnatural weak electric currents between human cells. In other words 24 hours a day if you live and sleep in a modern home. [1] These unnatural currents in the body are the direct result of the body being insulated from ground contact[F10]. The question is do these currents along with the static electricity created on the body from carpets etc. interfere with normal bioelectrical functions? An indication is; according to the American Institute of Stress, over 75% of all visits to primary care physicians are now for stress related health conditions. The description of stress is; a state of continuous anxiety and nervousness in which muscles become and remain tensed. Stress is now confirmed to be a primary contributor to cardiovascular disease, cancer, gastrointestinal, skin, neurological and emotional disorders, and a host of disorders linked to immune system disturbances ranging from the common cold and herpes, to arthritis[F11]. [2] In the late 1960s, when humans were first widely diagnosed as being stressed, synthetic soled shoes, carpets and the like had just become popular and the use electricity and household electrical devices tripled from the previous generation. Do these unnatural weak electric currents in the body also interfere with sleep? According to the National Sleep Foundation’s Sleep-2000 report [3], nearly two thirds of American adults [62%] now suffer from sleep problems. Americans have the most comfortable beds and the most protected sleep environments in the world. Yet, in traditional societies where most humans sleep on animal skins, grass mats or directly on the ground, sleep problems do not exist. [4] As for Americans, most now sleep within 12 inches of electrical wires hidden in the wall at the head of their bed and with electric cords around or near the bed. All of which emanate e-fields throughout the night and create weak electric currents in the body [1]. The fact that the majority of people, with the best health care in human history, now increasingly suffer from poor sleep and stress related health problems suggests that something, largely unknown to the health community and public, is wrong.[F12] The dramatic change from the body being naturally grounded to now conducting unnatural weak electric currents between cells is the most likely candidate. Circumstantial evidence is provided by the fact that humans in traditional societies that maintain contact with the earth do not experience the common sleep and stress related health problems of the modern world [F13][4]. Nor do the animals that live in direct contact with the earth. Evidence that is more conclusive was reported by the NIEHS and the DOE [1] that in some laboratory studies the biological effects of EMFs are: -Changes in functions of cells and tissue -Accelerated tumor growth -Decrease in the hormone melatonin -Changes in biorhythms -Alterations of immune system -Changes in human brain activity and heart rate The question is; by restoring natural ground to the body and thereby neutralizing these weak electric currents in the body and static electricity on the body, do muscle relax and normal sleep return? In search of the answer the following test was performed. METHOD AND MATERIALS -To effectively restore ground contact for an extended period, test subjects slept on dissipative carbon fiber mattress pads placed under their fitted sheets, connected via a ground wire [protected with an inline 1/100 amp fast blow fuse], to a ground rod driven into the earth near their bedroom window. The grounded mattress pads were designed to replicate the ground plane of the earth in the bed. Sleep disturbances along with chronic muscle and joint pain, which the subjects had been experiencing for at least six months, were recorded to establish a base line. The test was for a period of 30 days. SELECTION OF PARTICIPANTS An advertisement, distributed to ten beauty salons in Ventura County, CA solicited individuals experiencing sleep problems accompanied by tense muscles and/or chronic joint pain to participate in the study. Of the respondents, sixty individuals participated. Age of subjects was between 23 and 74 years Male subjects = 22 Female subjects = 38 Declared sleep problems = 100% Declared chronic muscle or joint pain = 100% The subjects were randomly divided into two groups. The first group of thirty, slept on carbon fiber mattress pads connected to a dedicated earth ground, just outside their bedroom window. The second control group of thirty, slept on carbon fiber mattress pads but were not connected to an earth ground. E-field created charge on their bodies were recorded with an AC voltmeter connected to the earth ground and body contact made with a hand held probe or an EKG electrode patch. E-field created charge measured on subject’s bodies while lying in their beds were as follows: Test subjects Control subjects Less than 1 volt 2 2 1 volts or more 28 28 2 volts or more * 16 15 3 volts or more 8 6 4 volts or more 4 3 5 volts or more 3 2 *All subjects averaged 2+ volts on their bodies while lying in their beds. E-field created charge measured on test subject’s bodies after grounding: averaged 10 millivolts or less.-
    Categories: Time to fall asleep Quality of sleep Wake feeling rested Muscles stiffness & pain Chronic back and/or joint pain General well-being Test Subjects* Same Improved 4=15% 23=85% 2=7% 25=93% 0=0% 27=100% 5=18% 22=82% 7=26% 20=74% 6=22% 21=78% Control Subjects** Same Improved 20=87% 3=13% 20=87% 3=13% 20=87% 3=13% 23=100% 0=0 % 23=100% 0=0% 20=87% 3=13%
    *Reports not received from three participants. **Reports not received from seven participants. DISCUSSION The purpose of this work was to provide evidence that when the human body is grounded it is naturally protected from static electricity and radiated electric fields. The meter reading of the grounded subject proved this true. The benefits of grounding the body were expected to relax muscles and improve sleep. This also proved to be true. Worthy of mention is that several subjects in the study stated they also experienced significant relief from asthmatic and respiratory conditions, rheumatoid arthritis, PMS, sleep apnea and hypertension, while sleeping grounded. These unexpected results indicate that loss of ground contact plays a much larger role in overall health then was anticipated at the start of this study. ADDITIONAL SUPPORT TO THESE FINDINGS In the May 1999 NIEH-EMF RAPID report, mention is made that reported biological effects to humans exposed to EMFs, such as changes in melatonin levels cannot be confirmed in animal studies. Therefore, the actual effects to humans are inconclusive. [5] In the animal studies, sheep exposed to EMFs from a power line were reported to have experienced no change in melatonin levels. The sheep, which walked and slept directly on the ground were naturally grounded throughout the experiment. The fact that melatonin levels remain normal in sheep when grounded, supports these findings that when humans are grounded their sleep improves.[F14]
    Personal note from Roger Coghill, MA Biol. MI Biol. MA Environ Mgt. a leading research scientist and author specialized in the field of Bioelectromagnetics, the science investigating the interaction of electricity with organic life.–Yes, I would be prepared to believe that grounding the body will help dispel any extraneous electric fields which could otherwise interfere with the body’s own endogenous fields. We have found that these latter are vital for wellbeing, with adverse effects if disturbed. It could also be a way forward for protection against high frequency radiation. Best, Roger Coghill 12/05/99- Comments from Russell Whitten, D.C. Ojai, CA 8/25/00
    Prompted by the results of a patient, who participated in Mr. Ober’s study, I grounded the beds of 35 additional patients over a two-month period. The bed e-field measurements in this group ranged from .3 to 47 volts before grounding. A variety of health benefits occurred in this time. Many of the improvements, such as increased energy and athletic performance, can be attributed to the improved sleep that almost everyone reported. However, in many cases metabolic and hormonal conditions responded as well. Chronic back pain went away in several cases, stiff arthritic joints became more flexible, asthma attacks subsided and PMS symptoms lessened greatly. These indications confirm that e -fields do affect the body. CONCLUSIONS The important finding of this study is that the human body when grounded is naturally protected from static electricity and the weak electric currents created in the body by radiated electric fields. The benefits of grounding the body are; sleep significantly improves, muscles relax, chronic back and joint pain subsides and general health improves. PRINCIPAL REFERENCES 1. National Institute of Environmental Health Sciences and the U.S. Department of Energy, Questions and Answers about EMF, electric and magnetic fields associated with use of electric power [1995] 2. American Institute of Stress, 3. National Sleep Foundation, 4. Slumbers Unexplored Landscape [1999] Carol M. Worthman, Anthropologist, Emory University Atlanta, GA 5. National Institute of Environmental Health Sciences EMF Rapid Report [May, 1999] For additional information on this study or for parties interested in performing additional studies related to personal grounding and health, please contact: Clint Ober @ 805- 844-0888 or e-mail clintober @