OMEGA 3….Danger and Misinformation
Fish oil won’t stop heart disease
Surprisingly, it was known back in 1979 that diet influenced EFA composition of the cell membrane; this finding was published in Cancer Research (1979; 39:1726-32).43 In 1990, a masterpiece of research conducted by William E. Lands found that the amount of critical parent omega-6 in the tissues was dependent on diet (Lipids 1990; 25(9):505-16).44To gain the best in scientific research, in 2002 I attended the world’s 1st Essential Fatty Acids and Human Nutrition and Health International Conference in Shanghai, China. There I discovered a shocking and unexpected discovery that fish oil lowers immunity. I nearly fell out of my chair! Overdosing on fish oil supplements can significantly decrease the effectiveness of your immune system, increasing your risk of contracting cancer. The International Society for the Study of Fatty Acids and Lipids (ISSFAL) June 2000 Congress in Tsukuba, Japan,45 had reported this startling fact, as noted earlier.And don’t think that fish oil prevents heart disease. It doesn’t. Cardiovascular Research (2002; 54:183-190) reported on a study where both the fish oil group and the control group showed close to equal atherosclerotic progression (arteries getting more clogged in spite of taking fish oil supplements). Nor did fish oil stop thickening of the artery. On the contrary, the artery wall got thicker (worsened) with fish oil ingestion! A mere 1.65 grams per day of fish oil supplement was taken-a great enough dose to cause adverse immunity and excessive internal bleeding, too.46 These results showing the failure of fish oil were published in 2002. Did this stop “experts” in the nutritional and medical fields and even in our governments from declaring how great fish oil supplements are? No! Harvard Medical School was involved in a study, published in 1995, titled “Controlled Trial of Fish Oil for Regression of Human Coronary Atherosclerosis” (Am Coll Cardiol 1995; 25(7):1492-8).47 The daily dose was six grams of fish oil versus six grams of olive oil in the control group. Their conclusion? “Fish oil treatment for two years does not promote major favorable changes in the diameter of atherosclerotic coronary arteries” (author’s emphasis). This means that arterial clogging was not decreased with the fish oil supplements.
Omega-6 derivative AA prevents blood clotting
Dr Warburg understood that slow blood speed allowed cancer to metastasise. Later, other researchers showed that if you can keep a localised cancer from metastasising, your risk of dying from cancer decreases by an amazing tenfold! Even though you may have cancer, you won’t die from it. Blood speed and viscosity have a connection to the spread of cancer. This is a surprising, seldom-mentioned fact that was pointed out by world-renowned molecular biologist Robert Weinberg.49 What causes metastasis? Blood clots, and this is known, too.50 What prevents blood from “sticking together” and is also Nature’s natural blood-thinner that prevents blood clots? No, it’s not omega-3, like you are constantly told. Parent omega-6 is much more powerful. Arachidonic acid (AA) is a critical omega-6 derivative and major biochemical component which occurs in virtually every cell we have. It is the building block of the most potent anti-aggregatory (“helps blood thinning”) agent known, termed prostacyclin. AA also inhibits platelet adhesion, making it a natural “blood thinner”. AA even helps solve vascular problems as a response to injury.51 Heart attack victims often have depleted EFA levels, especially the EFA derivatives AA from parent omega-6 and EPA from parent omega-3.56 We need some parent omega-3 because EPA is one of its important derivatives. The problem is that fish oil supplements overdose us with far too much.
What’s really clogging the arteries
Contrary to what we have heard for decades, it is not the saturated fat that clogs the arteries and impedes blood flow: it’s the adulterated parent omega-6. A groundbreaking Lancet article (1994; 344:1195-96) reported investigating the components of arterial plaques. Felton et al. measured the individual components, and in an aortic artery clog they found over 10 different compounds but no saturated fat.57 There was some cholesterol in the clog. This is explained by the fact that cholesterol acts as a protective healer for arterial cuts and bruises, just like a scab forms over external cuts. What is the predominant component of a clog? You probably guessed it: the adulterated omega-6 polyunsaturated oils-those that start out containing properly functioning EFAs but get ruined during commercial food processing. Many similar analyses of arterial clogs showing the same result have been carried out and published in the medical journals, but it would seem that few physicians have seen them.58 The average person has little, if any, chance of ever discovering the truth. So, it is not cholesterol itself that clogs the arteries. If you have a deficiency of EFAs, cholesterol acts as a “poison delivery system”. EFAs are cholesterol’s major component. As the medical textbook Molecular Biology of the Cell makes clear (p. 481), cholesterol is necessary for the structural integrity of the lipid bi-layer, the matrix in each of our 100 trillion cell membranes. JAMA (1994; 272:1335-40) published an article stating that cholesterol-lowering drugs do not work significantly to prevent heart disease. The reason? They can’t lower the amount of defective parent omega-6 enough. As stated in Current Atherosclerosis Reports (2004; 6:477-84), this is why cholesterol drugs can’t do the job:59 “LDL contains up to 80% lipids [fats and oils], including polyunsaturated fatty acids and cholesterol, mainly esters. Linoleic acid (LA), one of the most abundant fatty acids in LDLÉ” With this information, we see that it is what the cholesterol is transporting-the adulterated EFAs-that is the problem. An article in Human Nutrition: Clinical Nutrition (1984; 38C:245-260) further verifies that it is parent omega-6 that makes up most of the fatty acids in LDL and HDL cholesterol.60 Don’t let anyone ever tell you that natural fats are “bad”. One hundred trillion cells need lots of EFA-containing natural fats; in particular, lots of parent omega-6.If just a little of this parent omega-6 is defective, reducing its ability to absorb oxygen and perform other cellular functions, it acts as a direct cause of cancer as well as heart disease
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The Fallacy of Fish Oil
Article summary
The benefits of fish oil supplementation have been grossly overstated
Most of the studies showing fish oil benefits are short-term, lasting less than one year
The only fish oil study lasting more than four years showed an increase in heart disease and sudden death
Fish oil is highly unstable and vulnerable to oxidative damage
There’s no evidence that healthy people benefit from fish oil supplementation
Taking several grams of fish oil per day may be hazardous to your health
A new study was recently published showing that 3g/d of fish oil in patients with metabolic syndrome increased LDL levels and insulin resistance. — But this study isn’t alone in highlighting the potential risks of high-dose fish oil supplementation. Chris Masterjohn’s latest article on essential fatty acids, Precious yet Perilous, makes a compelling argument that fish oil supplementation – especially over the long-term – is not only not beneficial, but may be harmful.–This may come as a surprise to you, with all of the current media hoopla about the benefits of fish oil supplementation. Yet the vast majority of the studies done that have shown a benefit have been short-term, lasting less than one year. The only trial lasting more than four years, the DART 2 trial, showed that fish oil capsules actually increase the risk of heart disease and sudden death. —A 2004 Cochrane meta-analysis of trials lasting longer than six months suggests that the cardiovascular benefits of fish oil have been dramatically over-stated. They analyzed 79 trials overall, and pooled data from 48 trials that met their criteria. The only effect that could be distinguished from chance was a reduced risk of heart failure. Fish oil provided no reduction in total or cardiovascular mortality.
Too much fish oil can wreak havoc in your body—Omega-3 fatty acids are highly vulnerable to oxidative damage. When fat particles oxidize, they break down into smaller compounds, like malondialdehyde (MDA), that are dangerous because they damage proteins, DNA, and other important cellular structures.—A study by Mata et al demonstrated that oxidative damage increases as intake of omega-3 fat increases. The results of this study were summarized in the Perfect Health Diet, by Paul and Shou-Ching Jaminet:
Notice the clear increase in TBARS (a measure of oxidative damage of the LDL particle) with omega-3 fat. It’s important to note that this was only a 5-week trial. If it had gone on for longer than that, it’s likely the oxidative damage caused by omega-3 fats would have been even worse. This isn’t surprising if you understand the chemical composition of fats. Polyunsaturated fats (PUFA) are highly vulnerable to oxidative damage because they’re the only fatty acids that have two or more double bonds, and it’s the carbon that lies between the double bonds that is vulnerable to oxidation (as shown in the figure below):
Another thing worth noting, if you haven’t already, is that intake of saturated and monounsaturated fats does not increase oxidative damage by a significant amount. This is illustrated in both the table and the diagram above: saturated fats have no double bonds, which means they are well protected against oxidation. MUFA is slightly more vulnerable, since it does have one double bond, but not nearly as much as PUFA which has several double-bonds. —A randomized, double blind, placebo-controlled trial likewise showed that 6 grams per day of fish oil increased lipid peroxides and MDA in healthy men, regardless of whether they were supplemented with 900 IU of vitamin E. And consumption of fresh, non-oxidized DHA and EPA has been shown to increase markers of oxidative stress in rats.
Fish oil not as beneficial as commonly believed—To be fair, at least one review suggests that fish oil supplementation is beneficial in the short and even intermediate term. A recent meta-analysis of 11 trials lasting more than one year found that fish oil reduced the relative risk of cardiovascular death by 13 percent and the relative risk of death from any cause by 8 percent.—-But the effect seen in this review was mostly due to the GISSI and DART-1 trials. They found that fish oil may prevent arrhythmia in patients with chronic heart failure and patients who have recently survived a heart attack. —-However, there is no evidence that people other than those with arrhythmia and chronic heart failure benefit from taking fish oil or that doses higher than one gram of omega-3 fatty acids per day provide any benefit over smaller doses. And then there’s the rather disturbing result of the DART-2 trial, the only fish oil study lasting more than four years, showing an increase in heart disease and sudden death.—It’s logical to assume the effects of oxidative damage would take a while to manifest, and would increase as time goes on. That’s likely the reason we see some benefit in short- and intermediate-term studies (as n-3 displace n-6 in the tissues), but a declining and even opposite effect in the longer-term DART-2 trial (as increased total PUFA intake causes more oxidative damage).—
The danger of reductionist thinking in nutritional research—The current fish oil craze highlights the danger of isolated nutrient studies, which unfortunately is the focus of nutritional research today. Kuipers et al. eloquently described the risks of this approach in a recent paper: –The fish oil fatty acids EPA and DHA (and their derivatives), vitamin D (1,25-dihydroxyvitamin D) and vitamin A (retinoic acid) are examples of nutrients that act in concert, while each of these has multiple actions(7,8). —Consequently, the criteria for establishing optimum nutrient intakes via randomised controlled trials (RCT) with single nutrients at a given dose and with a single end point have serious limitations. They are usually based upon poorly researched dose–response relationships, and typically ignore many possible nutrient interactions and metabolic interrelationships. —For instance, the adequate intake of linoleic acid (LA) to prevent LA deficiency depends on the concurrent intakes of α-linolenic acid (ALA), γ-LA and arachidonic acid (AA). Consequently, the nutritional balance on which our genome evolved is virtually impossible to determine using the reigning paradigm of ‘evidence-based medicine’ with RCT. –Interest in fish oil supplementation started with observations that the Inuit had almost no heart disease. It was assumed their high intake of marine oils produced this benefit. While this may be true, at least in part, what was overlooked is that the Inuit don’t consume marine oils in isolation. They eat them as part of a whole-food diet that also includes other nutrients which may help prevent the oxidative damage that otherwise occurs with such a high intake of fragile, n-3 PUFA.—-It’s also important to note that there are many other traditional peoples, such as the Masai, the Tokelau, and the Kitavans, that are virtually free of heart disease but do not consume high amounts of marine oils. What these diets all share in common is not a large intake of omega-3 fats, but instead a complete absence of modern, refined foods.
Eat fish, not fish oil – cod liver oil excepted—That is why the best approach is to dramatically reduce intake of omega-6 fat, found in industrial seed oils and processed and refined foods, and then eat a nutrient-dense, whole-foods based diet that includes fatty fish, shellfish and organ meats[U1]. This mimics our ancestral diet and is the safest and most sane approach to meeting our omega-3 needs – which as Chris Masterjohn points out, are much lower than commonly assumed. –So I still recommend eating fatty fish a couple times per week. What I don’t endorse is taking several grams per day of fish oil, especially for an extended period of time. Unfortunately this advice is becoming more and more common in the nutrition world.–More is not always better, despite our tendency to believe it is
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Omega-3 contamination study is misleading, says industry
A new study on pollutant levels in fish oil supplements has been criticized by industry as being misleading and sensationalist. –Published this month in the Journal of Food Science, the study investigated levels of persistent organic pollutants (POPs) in 30 fish oil supplements found on the Canadian market. POPs, including polychlorinated biphenyls (PCBs) and organochlorine insecticide (OC) compounds, have been linked to immunotoxicity and carcinogenicity. Differing levels of these contaminants have been detected in fish products from around the world. The current study, entitled Persistent Organic Pollutants in Fish Oil Supplements on the Canadian Market: Polychlorinated Biphenyls and Organochlorine Insecticides, examined fish oil supplements purchased in Vancouver, Canada, between 2005 and 2007.
Contamination levels –PCBs and OC insecticides were detected in all supplement samples collected. However, most of these levels did not surpass acceptable intake levels, prompting the omega-3 industry to criticize it as “misleading”. According to Ocean Nutrition Canada, a leading manufacturer of omega-3 fish oil ingredients used in foods and supplements, the paper has “a sensational headline that does not reflect the study’s findings and conclusions”. This, said the firm, could generate confusion in the way the information is communicated to the public. The researchers – from Health Canada, Bureau of Chemical Safety and Food Directorate – tested both single-species oil supplements (such as salmon, seal or shark), as well as mixed oil products (for example salmon, anchovy, sardine and mackerel). Their findings revealed PCB levels ranging between 0.711 ng/g (equivalent to 0.711 ppb) in a mixed oil product and 10,400 ng/g (10,400 ppb) in a shark oil product. In Canada, the acceptable limit of PCBs is 2,000 ppb, placing the shark oil product far beyond the limit. The second highest level of PCBs was 519 ng/g, found in seal oil. “With the exception of the menhaden, seal, and shark oil supplements, all median ΣPCB concentrations were below 100ng/g,” notes the study. OC levels in oil supplement samples also varied significantly. However, none of the oils exceeded the acceptable limit of DDT – an OC insecticide – which is 5,000 ppb. The maximum concentration of most OC insecticides were found in the seal oil analyzed. The supplements containing seal, shark, and salmon oils had elevated levels of OC compounds relative to those containing other fish and vegetable oils. Mixed oils (anchovy, mackerel and sardine) had the lowest level of DDT at 0.189 to 15.2 ppb. “The mixed fish oils tested in the present study had lower PCB and OC insecticide levels than other oil types. Although a limited number of supplements were analyzed in this study, variable concentrations of PCBs and OCs were observed corresponding to oil type,” the researchers concluded.
Vegetarian fish
Ocean Nutrition highlighted that seal oil and shark oil – which were found to have the highest contaminant levels – are not an accurate representation of omega-3 oils. “Fish oil primarily is sourced from the Peruvian Anchovy fishery. These fish are low on the food chain and are vegetarians, primarily consuming algae. This means they are very pure from contaminants as a starting source, compared to species such as seals and shark, which are high on the food chain and bi accumulate contaminants,” the company told NutraIngredients-USA.com. The Global Organization for EPA and DHA (GOED), an omega-3 trade association, also said that seal and shark oils are “obscure products that are actually difficult to find”. The group’s executive director Adam Ismail noted that these products are outside the scope of the GOED voluntary monograph, which sets quality standards for fish oils. “The other thing the study highlights is that in many instances omega-3 supplements can be a safer source of EPA and DHA than actual fish consumption, with a single serving of salmon containing more than 100 times the PCBs than the standard fish oil supplements used in the study, and 30 times more than the limits in the monograph,” he said.
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Omega-3 doesn’t help heart attack patients, say German researchers
01-Apr-2009
University of Heidelberg researchers in Germany have found heart attack patients don’t benefit from omega-3 supplementation in a randomised study involving about 4000 post-heart attack patients. –Their paper, presented at the 58th conference of the American College of Cardiology, found fish oil omega-3 supplements had no more benefit for patients already taking pharmaceuticals for heart conditions, than placebo. —Despite the apparently negative results, the researchers did not condemn fish oil supplementation, noting the study did not have as an aim, whether fish oil can prevent the onset of heart disease in the first place. —The American Heart Association recommends that coronary heart disease patients take a gram of omega-3s per day, and recommends 2 to 4 grams per day to patients needing to lower triglyceride levels. –The high-dose omega-3, prescription form used in the trial is sold as Omacor and Lovaza in the US and Zodin in Europe.
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Vitamin E –Benefits and therapeutic process
Natural Alpha Tocopherol (Vitamin E) in the treatment of Cardiovascular and Renal Diseases as suggested by Drs. Wilfrid and Evan Shute and the Shute Institute for Clinical and Laboratory Medicine, London, Ontario, Canada. Use only products labeled in terms of InternatIonal Units (IU).
Acute coronary thrombosis: 450 to 1,600 IU a day started as soon as possible and maintained.
Older cases of coronary thrombosis: 450 to 1,600 IU if systolic pressure is under 160 Otherwise 450 IU for the first four weeks, particularly if a hypotensive agent is used concurrently.
Acute rheumatic fever: 450 to 600 IU daily.
Chronic rheumatic heart disease: give 90 IU daily first month, 120 IU daily second month and 150 IU daily for third month. 150 IU may be ideal dose. Occasionally more is necessary and advisable. Response will necessarily be slow.
Anginal Syndrome: 450 to 1,600 IU if systolic pressure is under 160. Otherwise start on 150 IU for four weeks then 300 IU for four weeks, particularly if hypotensive agent is used.
Hypertensive heart disease: 75 IU daily for four weeks, 150 IU daily for four weeks, then cautiously increase. Should be used with hypotensive agents. High doses of vitamin E have been shown to reduce high blood pressure in rats with chronic kidney failure. (Vaziri N. Hypertension, Jan 2002.)
Thrombophlebitis and Phlebothrombosis: 600 to 1,600 IU daily.
Thrombocytopaenic Purpura: 800 to 1,200 IU daily.
Diabetes Mellitus: Same schedule as for cardiacs.
Acute and Chronic Nephritis: as for cardiac patients.
Burns, Plastic Surgery, Mazoplasia: 600 to 1,600 IU daily, using vitamin E ointment or vitamin E spray as adjunct. (Editor’s note: vitamin E may also be dripped from a thumbtack-punctured capsule.)
CAUTIONS
The maintenance dose equals the therapeutic dose.
Do not take iron and vitamin E at same time. If iron is indicated, separate the doses by about nine hours.
The digitalis requirement is often reduced after vitamin E takes hold, so over-digitalization should be avoided. A patient receiving vitamin E should not be digitalized by the Eggleston massive dose technique nor any of its modifications. It is usually sufficient for full digitalization to give what is ordinarily a maintenance dose of 1 1/2 grains digitalis folia or 0.1 mg digitoxin per day. By the second day the patient is often digitalized.
Insulin dosages in diabetic cardiacs must be watched closely, for the insulin requirement may be considerably reduced very suddenly.
Hyperthyroidism is sometimes a contraindication.
Estrogens should rarely be given at the same time as alpha tocopherol (vitamin E).
(Editor’s note: The Shutes also recommend caution with patients who have untreated high blood pressure, a rheumatic heart, or congestive heart failure. If you are a person with these or any other preexisting medical condition, you need to WORK WITH YOUR PHYSICIAN TO DETERMINE YOUR OPTIMUM VITAMIN E LEVEL.)
TWELVE EFFECTS OF ALPHA TOCOPHEROL (Vitamin E)
1. It reduces the oxygen requirement of tissues.
Hove, Hickman, and Harris (1945) Arch. Biochem. 8:395.
Telford et al (1954) Air University School of Aviation Medicine Project #21-1201-0013, Report #4, May. Randolph Field, Texas.
2. It melts fresh clots, and prevents embolism.
Shute, Vogelsang, Skelton and Shute (1948) Surg., Gyn. and Obst. 86:1.
Wilson and Parry (1954) Lancet 1:486.
3. It improves collateral circulation.
Enria and Fererro (1951) Arch. per Ia Scienze Med. 91:23.
Domingues and Dominguez (1953) Angiologia 5:51.
4. It is a vasodilator.
Shute, Vogelsang, Skelton and Shute (1948) Surg., Gyn. and Obst. 86:1.
5. It occasionally lyses scar tissue.
Steinberg (1948) Med. Clin. N. America 30:221, 1946.
6. It prevents scar contraction as wounds heal.
Shute, Vogelsang, Skelton and Shute (1948) Surg., Gyn. and Obst. 86:1.
7. It increases low platelet counts.
SkeIton, Shute, Skinner and Waud (1946) Science 103:762.
8. It decreases the insulin requirement in about 1/4 of diabetics.
Butturini (1950) Gior. di Clin. Med. 31:1.
Tolgyes (1957) Summary 9:10.
9. It is one of the regulators of fat and protein metabolism.
Hickman (1948) Rec. of Chem. Progress, p.104.
10. It stimulates muscle power.
Percival (1951) Summary 3:55.
11. It preserves capillary walls.
Ames, Baxter and Griffith (1951) International Review of Vitamin Research 22:401.
12. It prevents haemolysis of red blood cells.
Rose and Gyorgy (1951) Fed. Proc.10:239. 1951.
OTHER RELEVANT PUBLICATIONS
Tolgyes, S. and Shute, E. V. (1957), Alpha Tocopherol in the Management of Small Areas of Gangrene. Can. M. A. J. 76:730.
Shute, E.V. (1957) The Prevention of Congenital Anomalies in the Human: Experiences with Alpha Tocopherol as a Prophylactic Measure. J. Ob. & Gyn. Brit. Emp. 44:390.
Hauch, J. T. (1957) A New Treatment for Resistant Pressure Sores. Can. M.A.J. 77:125.
Shute, E. V. (1957) Alpha Tocopherol in Cardiovascular Disease. Oxford University Med. Gaz. 9:96.
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[U1]These days I would disagree with this inclusion of foods since these foods are either bottom feeders or organ meats which filter out the heavy poisons in our environment –this should be avoided since most people are already overloaded with poison–
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Show of the Week November 8, 2010
Accessory to a criminal Practice—Nutrition House
Apoptotic effect of eugenol in human colon cancer cell lines
Remedy for a blocked stomach
Half of Those Travelling Internationally Not Aware of Potential Health Risks, Study Finds
Vaccines Could Help What’s Ailing Fish
What in the World Are They Spraying
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Accessory to a criminal Practice—Nutrition House
As the regulations start to come into effect , the inspectors will be looking for specific products that have been deemed illegal in Canada. These products have long since been unapproved for sale here but some stores try to sneak them in. Please do not be one of these stores. Nutrition house is working with Health Canada to ensure that stores that carry these products are brought to light. If we cannot sell them no store should. Please be sure to clean up your store and if you have these products , under the counter or elsewhere get rid of them. Keep this email in mind as there will be industry rumours as to what happened.
If you have any questions please feel free to contact me-Catherine
Health supplements seized over safety
Last Updated: Thursday, November 4, 2010 | 3:10 PM ET Comments0Recommend0
CBC News
A number of supplements targeted at exercise buffs have been seized in raids on two fitness facilities in British Columbia. Health Canada inspectors collected 18 varieties of supplements from Fitness Etc. locations in Courtney and Campbell River on Vancouver Island. The public health agency said the supplements were removed because they contain ingredients that legally require a prescription. Many also contain a combination of ephedra and caffeine, which was banned in Canada in 2002. The combination was found to cause serious heart problems that in some cases led to death. Some of the seized supplements also contain M1T or DHEA, which is a steroid and sex hormone precursor that is regulated as a controlled drug in Canada. It cannot be purchased without a prescription.
Health Canada is now working with Canada Border Services to stop the importation of the unauthorized health supplements. — Read more: http://www.cbc.ca/health/story/2010/11/04/con-supplements-seized.html?ref=rss#ixzz14LPvJ1t9
Barbara Laidlaw
Senior Vice President
Fleishman-Hillard Canada
33 Bloor Street East, Suite 1500
Toronto, ON M4W 3H1
Office direct: (416) 645-3666
Mobile: (416) 704-4742—www.fleishman.ca
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Apoptotic effect of eugenol in human colon cancer cell lines.
Cell Biol Int. 2010 Nov 2;
Authors: Jaganathan SK, Mazumdar A, Mondhe D, Mandal M
Eugenol, a natural compound available in honey and various plants extracts including cloves and Magnoliae Flos, is exploited for various medicinal applications. Since most of the drugs used in the cancer are apoptotic inducers, the apoptotic effect and anticancer mechanism of eugenol were investigated against colon cancer cells. Antiproliferative effect was estimated using 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyl tetrazolium bromide assay (MTT). Earlier events like mitochondrial membrane potential (MMP), thiol depletion and lipid layer break were measured by using flow cytometry. Apoptosis was evaluated using Propidium Iodide (PI) staining, Terminal deoxynucleotidyl transferase (TdT) mediated dUTP nick end labelling (TUNEL) assay and DNA fragmentation assay. MTT assay signified the antiproliferative nature of eugenol against the tested colon cancer cells. PI staining indicated increasing accumulation of cells at sub-G1 phase. It was 33.29 ± 4.93 and 37.84 ± 4.28 for HT-29 and HCT-15 after 48 h for eugenol treated cells. Eugenol treatment resulted in reduction of intracellular non-protein thiols and increase in the earlier lipid layer break. Further events like dissipation of MMP and generation of reactive oxygen species (ROS) were accompanied in the eugenol-induced apoptosis. Augmented ROS generation resulted in the DNA fragmentation of treated cells as shown by DNA fragmentation and TUNEL assay. Further activation of poly-adenosine diphosphate-ribose polymerase (PARP), p53 and caspase-3 were observed in the western blot analyses. Our results demonstrated molecular mechanism of eugenol-induced apoptosis in human colon cancer cells. This research will further enhance eugenol as a potential chemopreventive agent against colon cancer.
PMID: 21044050 [PubMed – as supplied by publisher]
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Remedy for a blocked stomach
If you have eaten things that are really hard to digest late at night-Like walnuts or pecans or something with a heavy fat and wake the next day with a belly ache—here is a remedy that may assist Using sea salt and and cream of tartar and 3 ounces of water out in equal amounts of salt and cream of tartar and drink this Add HCL pills if you have them 1 caplet or capsule Then use a light oil like almond—2 teaspoons And then walk for 2 minutes and then lay down for about 5— And the rest of the day make a gelatin broth with a light oil ( almond or sunflower-or apricot – and do this broth mix ( 4-5 ounces ) and consume this By days end it will be clear and you will have passed the obstruction This will lubricate the colon and intestinal trac as well as regenerate the damage with the gelatin
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Half of Those Travelling Internationally Not Aware of Potential Health Risks
ScienceDaily (Nov. 3, 2010) — More than 30 million people in the United States travel to resource-limited areas of the world each year. This global mobility may contribute to the spread of infectious diseases — such as influenza, measles, and meningitis — and may also put individual travelers at risk for malaria, typhoid, dengue fever and hepatitis. Despite these potential risks, a recent study conducted by the Division of Infectious Diseases at Massachusetts General Hospital (MGH) and published in the Journal of Travel Medicine found that 46 percent of travelers to resource-limited countries did not seek health advice or vaccinations prior to departure.—The researchers surveyed more than 1,200 international travelers departing the United States at Boston Logan International Airport. The study was the result of a broad-based collaboration between MGH, the Centers for Disease Control and Prevention (CDC), the Boston Public Health Commission, and officials from the Massachusetts Port Authority, which owns and operates Logan International Airport. Based on the results from this work, the CDC, travel medicine experts and Logan Airport officials hope to develop better tools to educate people about the public health risks associated with global travel.—Of those surveyed, 38 percent were traveling to countries described as low- and low-middle income by the World Bank’s World Development Report, yet 46 percent of those travelers did not seek health advice prior to departure. Foreign-born travelers — including those traveling to visit family and friends, and those traveling alone or for vacation — were the least likely to have researched health information. The most commonly cited reason for not pursuing health information was a lack of concern about potential health problems.—Of the 54 percent of travelers to resource-limited countries who did seek health information, the Internet was the most common source, followed by primary care practitioners (PCPs).—“These results suggest that the Internet and PCPs are two promising avenues for disseminating information about traveling safely,” says the study’s lead author Regina C. LaRocque, MD, MPH, of MGH’s Division of Infectious Diseases. “Offering online resources at the time of ticket purchase or through popular travel websites would likely reach a large audience of people in need of health advice.”–The rapid global spread of Severe Acute Respiratory Syndrome (SARS) in 2002-2003 and new influenza strains in 2009 exemplified the role played by travelers in disseminating infectious diseases. More recently, dengue fever — a tropical disease found mainly in the Caribbean, Latin America and Asia — has been reported in the southern United States. In India, an epidemic of Chikungunya — a viral infection characterized by fever, headache, weakness and joint pain — was spread to Italy by travelers.—“International travel is the primary way many infections traverse the world,” says Edward Ryan, MD, director of the Tropical and Geographic Medicine Center in the Division of Infectious Diseases at MGH and a senior author of the study. “What many people don’t realize is that, without seeking the correct health information, they are putting themselves at increased risk of infection, as well as creating a public health risk in their home communities after they return.”—-This study was funded by CDC grants aimed at gathering demographic data on international travelers. Additional ongoing research on U.S. international travelers is being conducted through the Global TravEpiNet, a consortium of travel clinics coordinated by MGH that is focused on improving research and health information regarding international travel.—Ryan is an associate professor and LaRocque an assistant professor of Medicine at Harvard Medical School. Additional authors of the Journal of Travel Medicine study are Sowmya R. Rao, PhD, Athe Tsibris, MD, and Thomas Lawton of the Massachusetts General Hospital; M. Anita Barry, MD, MPH, of the Boston Public Health Commission; and Nina Marano, DVM, MPH, Gary Brunette, MD, and Emad Yanni, MD, of the Centers for Disease Control and Prevention.—Story Source:–The above story is reprinted (with editorial adaptations by ScienceDaily staff) from materials provided by Massachusetts General Hospital, via EurekAlert!, a service of AAAS.—Journal Reference: Regina C. LaRocque, Sowmya R. Rao, Athe Tsibris, Thomas Lawton, M. Anita Barry, Nina Marano, Gary Brunette, Emad Yanni and Edward T. Ryan. Pre-travel Health Advice-Seeking Behavior Among US International Travelers Departing From Boston Logan International Airport. Journal of Travel Medicine, 2010; 17 (6): 387-391 DOI: 10.1111/j.1708-8305.2010.00457.x
ØSuggested things to take with you when you travel: Lugols Iodine- GSE- Bee Propolis or Balm of Gilead-Digestive enzymes and MSM—when out of the normal parameter of your environment use wine or spirits to maintain a clean inside–And Vitamin C and Zinc and Selenium in a supplemental form–this will minimize getting anything or at the least mitigate or lessen the severity
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Vaccines Could Help What’s Ailing Fish
Immersion vaccines have been used for decades, but current work on developing alternative methods of vaccine delivery through feeding is promising. Here, molecular biologist Craig Shoemaker (left) and microbiologist Phillip Klesius demonstrate a technique where channel catfish are immersed in water containing the modified live Streptococcusiniae vaccine—ScienceDaily (Oct. 26, 2010) — U.S. Department of Agriculture (USDA) scientists are developing vaccines to help protect healthy farm-raised catfish against key diseases.–Working as a team, microbiologist Phillip H. Klesius and molecular biologists Julia Pridgeon and Craig Shoemaker with USDA’s Agricultural Research Service (ARS) at the agency’s Aquatic Animal Health Research Unit in Auburn, Ala., and Joyce J. Evans, aquatic pathologist at the Auburn unit’s lab in Chestertown, Md., are developing vaccines against Streptococcus iniae, S. agalactiae and other pathogens.–ARS is USDA’s principal intramural scientific research agency. This research supports the USDA priority of promoting international food security.
The scientists modify the genetic makeup of pathogens to make them nonvirulent, and then develop vaccines that expose fish to low doses of the modified forms of the pathogens.–Klesius and Pridgeon have developed a modified live S. iniae vaccine that appears to be superior to inactivated or killed vaccines. The live modified vaccine has enough similarity with the pathogen to create a lifelong immunity in fish, according to Klesius.—Scientists are looking at new methods to vaccinate fish. But for now, the vaccination process consists of immersing the fish in water that contains the modified pathogen.–Previous research breakthroughs have benefited the catfish industry. For example, a ARS-developed vaccine against the pathogen Edwardsiella ictaluri, which causes enteric septicemia, has been widely adopted by fish growers.—In an earlier trial, the vaccine against enteric septicemia of catfish was tested by Mississippi State University researchers. Results showed a 12 percent increase in the survival rate of fish that were given the vaccine, and a substantial increase in returns for producers who used the vaccine in their ponds.—Read more about this and other research on aquaculture in the October 2010 issue of Agricultural Research magazine at: — http://www.ars.usda.gov/is/AR/archive/oct10/fish1010.htm — Story Source: The above story is reprinted (with editorial adaptations by ScienceDaily staff) from materials provided by USDA/Agricultural Research Service. The original article was written by Sandra Avant.
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“What in the World Are They Spraying”
Produced by G. Edward Griffin, Michael Murphy, and Paul Wittenberger
Video Presentation and Discussion Group
Sat., Nov 20 1PM-5PM
Mesquite Public Library
4525 E. Paradise Village Pkwy. North
Phoenix, Arizona 85032-6853
Limited Seating RSVP
Samantha.kent.2000@gmail.com
(602-463-6367)
Presented by: Arizona Agriculture Defense Coalition
The story of a rapidly developing industry called geo-engineering.
Public participation is encouraged to join the discussion.
Attendees will receive important information including the DVD produced by Dr. Michael Coffman, PhD~”Global Warming, Emerging Science and Understanding” also DVDs of What in the World Are They Spraying
We look forward to meeting with all of you who share our concern.
All good things happen when we each begin to act on what is true and right.
Today is a good day to begin.
Mike Caraway & Bridget Conroy
Tools of Light
